We narrowed to 12,476 results for: 109

1. pX165-Sniper-Cas9

   Plasmid

   #140560

   Purpose

   Expresses Sniper Cas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   F539S M763I K890N

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTopo pLox hPGK-Puro pA pLox

   Plasmid

   #171048

   Purpose

   Empty donor template for CRISPR/Cas9 targeting of TERT RE's

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. JP1520 PIK3CA E545K HA

   Plasmid

   #14571

   Depositor

   Joan Brugge

   Article

   Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

   Insert

   PIK3CA E545K (PIK3CA Bovine)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   E545K

   Promoter

   Available since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pU6-TREX1 g1 Cas9-T2A-mCherry

   Plasmid

   #164250

   Purpose

   Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgTREX1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. cavin-3-HA

   Plasmid

   #27711

   Depositor

   Ari Helenius

   Article

   Hayer et al Traffic. 2010 Mar . 11(3):361-82.

   Insert

   cavin-3 (CAVIN3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R8P

   Promoter

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. BAT8 (3RJW)

   Plasmid

   #28125

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   BAT8 (EHMT2 Human)
   

   Use

   Tags

   His-tag with integrated thrombin site

   Expression

   Bacterial

   Mutation

   contains amino acid residues 913-1193.

   Promoter

   Available since

   Feb. 10, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pSTAP-seq_human_spike-in

   Plasmid

   #125152

   Purpose

   Empty vector to clone and measure the expression of promoter candidates (spike-in) by determining the abundance of transcripts originating from the candidate in human cells

   Depositor

   Alexander Stark

   Article

   Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

   Type

   Empty backbone

   Use

   Stap-seq screening vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BRAF - A12G in pENTR

   Plasmid

   #216606

   Purpose

   gateway cloning donor vector containing BRAF ASD mutation

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   BRAF (BRAF Human)
   

   Use

   Gateway entry plasmid

   Tags

   Expression

   Mutation

   A12G

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET41-GST-KaiC

   Plasmid

   #68051

   Purpose

   Strain: AM5117, Used for protein purification

   Depositor

   Susan Golden

   Article

   Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

   Insert

   KaiC
   

   Use

   Tags

   6xHis, GST, and S-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. N-WASP (WGP)

    Plasmid

    #11597

    Depositor

    Marc Kirschner

    Article

    Rohatgi et al J Cell Biol. 2000 Sep 18. 150(6):1299-31

    Insert

    N-WASP (Iqub Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains amino acids 1-396

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pQCXIZ 3xFLAG-TREX1

    Plasmid

    #164243

    Purpose

    retroviral plasmid for 3xFLAG-TREX1

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pExo1

    Plasmid

    #53357

    Purpose

    Expresses beta-lactamase for the degradation of beta-lactam antibiotics and tags it with hlyAs (transporter gene) in preparation for secretion from the cell.

    Depositor

    Lingchong You

    Article

    Pai et al Proc Natl Acad Sci U S A. 2012 Nov 27;10

    Inserts

    BlaMs
    hlyAs
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    p(lac/ara-1)

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCyclinB1(L45A,R42A)-mCherry (1963)

    Plasmid

    #39852

    Depositor

    Jonathon Pines

    Article

    Gavet et al J Cell Biol. 2010 Apr 19;189(2):247-59.

    Insert

    CyclinB1 (CCNB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    L45A and R42A

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

14. JP1520 PIK3CA H1047R HA

    Plasmid

    #14572

    Depositor

    Joan Brugge

    Article

    Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

    Insert

    PIK3CA H1047R (PIK3CA Bovine)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    Available since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pET28-SUMO-KaiB

    Plasmid

    #68050

    Purpose

    Strain: AM5116, Used for protein purification

    Depositor

    Susan Golden

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

    Insert

    KaiB
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET29b_Qcat18

    Plasmid

    #45146

    Depositor

    Christopher Petzold

    Article

    Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

    Insert

    E-coli-pathways-QconCAT18
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX165-xCas9

    Plasmid

    #140568

    Purpose

    Expresses xCas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    A262T R324L S409I E480K E543D M694I E1219V

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28-SUMO-KaiA

    Plasmid

    #68049

    Purpose

    Strain: AM5115, Used for protein purification

    Depositor

    Susan Golden

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

    Insert

    KaiA
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFPC1/GgVcl 1-1066

    Plasmid

    #46265

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

    Insert

    Vcl (VCL Chicken)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pShGSK3bE

    Plasmid

    #37526

    Depositor

    Jerry Crabtree

    Article

    Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

    Insert

    GSK3b (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SRa promoter

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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