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pAFZifPZ431

Plasmid

#185849

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>ZifPZ43>MED3>PABF1

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pXL-BACII-attPGAL4LWL

Plasmid

#26260

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pBAV-B

Plasmid

#65928

Purpose

Theophylline Riboswitch B with lacZ gene

Depositor

Justin Gallivan

Article

Topp et al Appl Environ Microbiol. 2010 Dec;76(23):

Insert

lacZ

Expression

Bacterial

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
XW54

Plasmid

#65839

Purpose

Punc-4c-NLS-QF-BD-DM-Nzipper-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

NLS-QF-BD-DM-Zipper

Tags

mCherry

Expression

Worm

Promoter

Punc-4c

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-CaaX(tH)

Plasmid

#109539

Purpose

Multisite gateway vector for 3' tagging with the CaaX domain from Hras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX domain from Hras

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-NS

Plasmid

#140874

Purpose

Multisite Gateway middle entry vector for fusing mScarlet to the 5' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Rca_Cas13a_WT

Plasmid

#91870

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

RcaCas13a (RcaC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pPP378

Plasmid

#158797

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-1 (D371A)

Expression

Bacterial

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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XW99

Plasmid

#65842

Purpose

MosSCI-Pmig-13::QS::SL2::mCherry for ttTi4348 on Chrom I.

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Neurospora crassa OR74A repressor (qa-1S)

Tags

mCherry

Expression

Worm

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-perox-mCer

Plasmid

#185603

Purpose

Lentiviral construct for expression of mCerulean targeted to peroxisomes

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-mCer

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc his Su(fu) (CT#768)

Plasmid

#13855

Depositor

Cliff Tabin

Article

Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

Insert

Su(fu) (SUFU Chicken)

Tags

his and myc

Expression

Mammalian

Mutation

full ORF

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pQE30-AtAEP2

Plasmid

#159529

Purpose

Expresses Arabidopsis thaliana asparaginyl endopeptidase 2 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Arabidopsis thaliana asparaginyl endopeptidase 2

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pQE30-CeAEP1

Plasmid

#159528

Purpose

Expresses jack bean (Canavalia ensiformis) asparaginyl endopeptidase 1 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Canavalia ensiformis asparaginyl endopeptidase 1

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pRK1036

Plasmid

#17728

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

NusG (nusG Aquifex aeolicus)

Tags

His6 and MBP

Expression

Bacterial

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pUC18-iSpinach

Plasmid

#186053

Purpose

template for co-transcriptional RNA folding assays

Depositor

Markus Wahl

Article

Huang et al Mol Cell. 2020 Sep 17;79(6):1024-1036.e5

Insert

T7A1-rrnG-iSpinach

Tags

no

Expression

Bacterial

Promoter

T7A1

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPP384

Plasmid

#158799

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-3 (D413A)

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBPHLWL-GAL4AD

Plasmid

#26255

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Gal4AD-Zip+ (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Expression

Bacterial

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Cam_Cas13a_WT

Plasmid

#91869

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

CamCas13a (CamC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Ere_Cas13a_WT

Plasmid

#91868

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

EreCas13a (EreC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-mCitrineBb

Plasmid

#118236

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCitrine

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mCitrine

Expression

Bacterial

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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