We narrowed to 22,610 results for: ESC;
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Plasmid#188516PurposeExpresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)DepositorInsertdCas12e
UseCRISPR and LentiviralTagsNLS-FLAG-VPRExpressionMammalianMutationD659A/E756A/D922APromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmRE-Tn5-142
Plasmid#118531PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FluoSTEP-AKAR (C/Y)
Plasmid#181862PurposeFluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AKAR (C-Y)
TagsCFP(1-10) and cpVenus(E127)ExpressionMammalianPromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPEE42
Plasmid#139421PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.DepositorInsertCitrine
ExpressionYeastPromoterTEF1Available SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEE45
Plasmid#139424PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.DepositorInsertmTurquoise1
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE44
Plasmid#139423PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end.DepositorInsertmTFP1
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE43
Plasmid#139422PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.DepositorInsertmMaroon1
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc C1A4E
Plasmid#141088Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of NanolucDepositorInsertAntares-T2A-puromycin-IRESv24-SEP-C1A4E
ExpressionMammalianMutationPlease see depositor commentsPromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA
Plasmid#133348Purposeexpression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)DepositorInsertsgRNA_blaA and sgRNA_cpaA
UseCRISPRPromoterconstitutiveAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b-GreBdm-D41N
Plasmid#129691Purposeexpress E. coli GreB E82C/C68S/D41NDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3
Plasmid#111922PurposeCyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μDepositorInsertCeNL(Ca2+)_1.2μ
TagsCoxVIIIx2ExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys-T46C
Plasmid#110543PurposeBacterial expression of E.coli DHFR C85A/C152S/T46C mutantDepositorInsertecDHFR
ExpressionBacterialMutationT46 mutated to C46, C85 mutated to A85, C152 muta…PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys-L54C
Plasmid#110542PurposeBacterial expression of E.coli DHFR C85A/C152S/L54C mutantDepositorInsertecDHFR
ExpressionBacterialMutationL54 mutated to C54, C85 mutated to A85, C152 muta…PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-D27S/Y100F
Plasmid#110827PurposeBacterial expression of E.coli DHFR D27S/Y100F mutantDepositorInsertecDHFR
ExpressionBacterialMutationD27 mutated to S27, Y100 mutated to F100PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ala-eK-Flv
Plasmid#83393Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ile-eK-Flv
Plasmid#83392Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Tyr-eK-Flv
Plasmid#83391Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0-GL-2-3 perfect copies
Plasmid#82441PurposeDual Fluorescent endogenous miRNA sensor Control insert. Three Perfect copies of complementary region of a siRNA control targeting firefly luciferase encoded by the pGL2DepositorInsertLuciferase
MutationThe complementary region for GL2 siRNA control wa…Available SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJOG250
Plasmid#80582Purposerecipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptIIDepositorInsertspnos:nptII-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI
UseCRISPRExpressionPlantMutationD10AAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJOG251
Plasmid#80583Purposerecipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTADepositorInsertspnos:PAT-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI
UseCRISPRExpressionPlantMutationD10AAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only