We narrowed to 18,889 results for: Tec

1. pLX-TRV1

   Plasmid

   #180515

   Purpose

   Agrobacterium tumefaciens-based expression of TRV1

   Depositor

   Jose-Antonio Daros

   Article

   Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

   Insert

   Tobacco rattle virus RNA1
   

   Expression

   Plant

   Promoter

   35S CaMV

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCKan-yibDp-mCherry

   Plasmid

   #127058

   Purpose

   Low phosphate inducible mCherry with N-terminal linker and His tag

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   mCherry
   

   Expression

   Bacterial

   Promoter

   yibDp

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a-LysRS-His

   Plasmid

   #124114

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Lysine-tRNA ligase (lysU Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2SC

   Plasmid

   #59382

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-cat
   

   Use

   Template

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 ZoAl N-1F

   Plasmid

   #203763

   Purpose

   For expression of N-flag tagged ZoAl R2 protein in mammalian cells

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement protein
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNCS YFP

   Plasmid

   #91759

   Purpose

   Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

   Insert

   Venus YFP (A206K)
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE30-His-AlaRS

   Plasmid

   #124103

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Alanyl-tRNA ligase (alaS Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6xHis-MBP-huTsCas12a

   Plasmid

   #174687

   Purpose

   Expresses 6xHis-MBP tagged TsCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huTsCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. act5C-mScarlet

   Plasmid

   #230906

   Purpose

   Ubiquitous expression of mScarlet in Drosophila melanogaster

   Depositor

   Michael Boutros

   Article

   Port et al bioRxiv 2024.11.26.625385

   Insert

   mScarlet
   

   Expression

   Insect

   Promoter

   act5C

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTK296

    Plasmid

    #59565

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PEST

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) 2xK-turn Kozak EGFP-PEST
    

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEC3135 (pERASE)

    Plasmid

    #220306

    Purpose

    Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG1344 EGFP-FIREtag

    Plasmid

    #205165

    Purpose

    Expresses EGFP-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    EGFP-FIREtag
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBT114

    Plasmid

    #138523

    Purpose

    phage cloning

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    phage genes
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a-AspRS-His

    Plasmid

    #124106

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Aspartate-tRNA ligase (aspS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a-ProRS-His

    Plasmid

    #124117

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Prolyl-tRNA ligase (proS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21a-TyrRS-His

    Plasmid

    #124121

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Tyrosyl-tRNA ligase (tyrS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-U6-epegRNA-SV40-mCherry

    Plasmid

    #240538

    Purpose

    Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with mCherry. The cloning site is BsmBI.

    Depositor

    Wensheng Wei

    Article

    Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

    Insert

    epegRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a-ValRS-His

    Plasmid

    #124122

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Valyl-tRNA ligase (valS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDonor14-N7HELIX-KanR

    Plasmid

    #190663

    Purpose

    Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Expression

    Bacterial

    Promoter

    NA

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBT29

    Plasmid

    #138521

    Purpose

    phage cloning

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    phage genes
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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