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pAAV-CaMKII-Ace-mNeon2

Plasmid

#195526

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

CaMKII

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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PB-Hyg-Dest-mCherry-PAR-6B

Plasmid

#194908

Purpose

To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

PAR-6B (Pard6b Mouse)

Use

Mouse Targeting

Tags

mCherry

Expression

Mammalian

Mutation

39 C to G, silent mutation

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

Plasmid

#163928

Purpose

Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

Plasmid

#191164

Purpose

Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:EGFP (additional cutsites)

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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APLF-c001

Plasmid

#173077

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

APLF (APLF Human)

Tags

mCerulean

Expression

Bacterial

Mutation

K360-V448, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-VARNAM2-Kv2.1PR

Plasmid

#195533

Purpose

Cre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMT-HuC:Dendra2

Plasmid

#80904

Purpose

Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2

Depositor

Periklis Pantazis

Article

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

Dendra2 (elavl3 Synthetic)

Use

Zebrafish expression

Promoter

HuC

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A1

Plasmid

#135503

Purpose

Mammalian expression of myc-tagged HLA-A*01:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*01:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pFUGW-GtACR1_C102A-EYFP

Plasmid

#119196

Purpose

Step-function mutant of Guillardia theta anion channelrhodopsin 1 (GtACR1) with dramatically prolonged open state

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin GtACR1 step-function mutant C102A

Use

Lentiviral

Tags

EYFP

Mutation

changed cysteine 102 to alanine

Promoter

Ubiquitin

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-VARNAM2-Kv2.1PR

Plasmid

#195536

Purpose

Flp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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XZ091 (PB) CMV-sCCL20 (PuroR-BFP)

Plasmid

#233434

Purpose

PiggyBac donor plasmid, secreted CCL20

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCL20 (CCL20 Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-VARNAM2-Kv2.1PR

Plasmid

#195530

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CaMKII

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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MacroH2A1.1-c002

Plasmid

#173087

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A1 (MACROH2A1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

Q162-N369, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Sema6c-AP-His

Plasmid

#72041

Purpose

Expresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6c (Sema6c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6d.4-AP-His

Plasmid

#72044

Purpose

Expresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.4 (Sema6d Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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MacroH2A1.2-c002

Plasmid

#173088

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A1 (MACROH2A1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

Q162-N372, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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ALC1-c005

Plasmid

#173092

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

CHD1L (CHD1L Human)

Tags

mCerulean

Expression

Bacterial

Mutation

L613-K879, codon optimized

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-pAceR-Kv2.1PR

Plasmid

#195525

Purpose

Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

Synapsin

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Plxnb1(L)-Fc-His

Plasmid

#72126

Purpose

Expresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb1 (Plxnb1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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ARH3-c002

Plasmid

#173095

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRS (ADPRS Human)

Tags

mCerulean

Expression

Bacterial

Mutation

A14-S363

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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