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Plasmid#195532PurposeCre-dependent expression of a green fluorescent, negative response-polarity voltage indicator; soma-targetedDepositorInsertAce-mNeon2-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterCAGAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
POLD1_pLX307
Plasmid#98358PurposeLentiviral expression of POLD1DepositorAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0 Tp
Plasmid#188619PurposeMutated pTA-Mob 2.0 Backbone in TraJ Promoter RegionDepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidExpressionBacterial and YeastAvailable SinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 HA-hTSPAN12
Plasmid#115784PurposeExpresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.DepositorInsertTSPAN12 (TSPAN12 Human)
TagsHAExpressionMammalianMutationmultiple silent mutations introduce restriction s…PromoterCMVAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZNF217_pLX307
Plasmid#98384PurposeLentiviral expression of ZNF217DepositorAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCS.DT-A
Plasmid#22730DepositorInsertDT-A
UseBac recombineeringMutationThis is a gap-repair vector designed for retrievi…Available SinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(S473D)
Plasmid#86577PurposeExpresses cleavable human full-length Akt1(S473D) phosphomimeticDepositorInsertAkt1 (AKT1 Human)
Tags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130; S473D phos…Available SinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Neo-EF1A>Tet3G
Plasmid#184379PurposeThe Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)DepositorInsertTet3G
UseLentiviralAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_cRed
Plasmid#117225PurposepET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.DepositorInsertCanthaxanthin Operon
Tags34 amino acid N-terminal linker, Hexahistidine Ta…ExpressionBacterialMutationremoved Type IIS restriction sites within the bac…PromoterT7 RNA PolymeraseAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CRISPRv2-sgNTC13-hygro
Plasmid#231988PurposeKnockout non-targeting controlDepositorInsertsgRNA with Cas9 and hygromycin resistance
UseCRISPR and LentiviralAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-GCaMP6s
Plasmid#183809Purposehis plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.DepositorInsertAAV transgene TetO promoter-DIO/flex-GCaMP6
UseAAV, Cre/Lox, and Mouse TargetingExpressionMammalianAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CRISPRv2-sgNTC49-puro
Plasmid#231989PurposeKnockout non-targeting controlDepositorInsertsgRNA with Cas9 and hygromycin resistance
UseCRISPR and LentiviralAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-pSFFV-TAF15N-mCherry-sspB
Plasmid#122436PurposeExpresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.DepositorInsertTAF15 (TAF15 Human)
UseLentiviralTagsmCherry and sspBExpressionMammalianMutationContains only amino acids 1-208PromoterSFFVAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1(A322D)-IRES2-EGFP
Plasmid#170821Purposehuman GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
TagsIRES2-EGFPExpressionMammalianMutationchange Alanine 322 to AspartateAvailable SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eGFP-NMHC-IIAdeltaIQ2
Plasmid#35690DepositorInsertMHC IIA (MYH9 Human)
TagsEGFP and GFPGenotRWExpressionMammalianMutationThe coding segment for aa 804-834 (corresponding …PromoterCMVAvailable SinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_PHC3
Plasmid#45262DepositorAvailable SinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-delStem1
Plasmid#177616PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseExpressionMammalianMutation3-nucleotide (UAC) deletion in Stem 1Available SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-neoBeta2mcherry
Plasmid#45097DepositorInsertnAChR beta2 mcherry (Chrnb2 Mouse)
Tagshas c-myc tagExpressionMammalianMutationβ2 was cloned into pCI-neo at the restriction sit…PromoterCMVAvailable SinceJune 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
TCR012H
Plasmid#233454PurposeExpresses ncHLAp-reactive T cell receptor (TCR012H)DepositorInsertTCR012H
UseLentiviralAvailable SinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pODN-AAVS1-mNG-CAAX
Plasmid#183870PurposeRepair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.DepositorInsertAAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
UseCRISPR; Donor templateTagsmNeonGreen-CAAXExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterCMVAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only