We narrowed to 6,260 results for: pcDNA 3.1

Showing: 4591 - 4620 of 6260 results

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YFP-DHFR-myc

Plasmid

#118852

Purpose

For fluorophore translocation assay and inducible tagging with zapalog.

Depositor

Thomas Schwarz

Article

Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

Type

Empty backbone

Tags

DHFR, Myc, and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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TRPML3-HA

Plasmid

#18832

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
Bongwoori-Pos6

Plasmid

#101655

Purpose

Bongwoori with the CC1-Pos6 linker length and composition.(* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

Bongwoori-Pos6

Expression

Mammalian

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_mCherry_2A_HA_2xMS2-VP64

Plasmid

#68419

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

MS2

Use

Synthetic Biology

Tags

HA and VP64

Expression

Mammalian

Mutation

MS2: V75E/A81G

Promoter

CMV

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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Cenp-B INCENP GFP

Plasmid

#45238

Purpose

Target INCENP to centromeres via full length CENP-B

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B, INCENP-Δcen, GFP

Tags

GFP and full length CENP-B

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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myr-palm_mCherry2-mEGFP

Plasmid

#127816

Purpose

Hetero-dimer mCherry2-mEGFP expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

myristoylated-palmitoylated-mCherry2-mEGFP

Expression

Mammalian

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Myc-hZO-1-HA

Plasmid

#236528

Purpose

For expression of ZO-1 (human) in mammalian cells

Depositor

Sandra Citi

Article

Spadaro et al Curr Biol. 2017 Dec 18;27(24):3783-3795.

Insert

TJP1 (TJP1 Human)

Tags

HA and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-mito-CitroffRH

Plasmid

#158548

Purpose

Mitochondria-targeted nonbinding variant of inverse-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

CitroffRH

Expression

Mammalian

Promoter

cmv

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pZ147-BvCas12b-sgRNA-scaffold

Plasmid

#122447

Purpose

Expresses sgRNA for BvCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

U6-BvCas12b-sgRNA-scaffold, CMV-mCherry

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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TR therapy reporter

Plasmid

#179083

Purpose

Fluorescent reporter for alternative splicing

Depositor

Thurman Wheeler

Article

Hu et al Nat Commun. 2018 Dec 7;9(1):5227. doi: 1

Inserts

DsRed1
EGFP

Expression

Mammalian

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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LacI INCENP

Plasmid

#45236

Purpose

Target INCENP to lac operator sequence

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

LacI, INCENP-Δcen, mCherry (lacI )

Tags

LacI (lac repressor protein) and mCherry

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Promoter

t7

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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p176 Grg-1s Myc His

Plasmid

#11065

Depositor

Ramesh Shivdasani

Article

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-S (Tle1 Mouse)

Tags

His and Myc

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pFH2.20_mCherry-MS2x4

Plasmid

#205535

Purpose

Express mCherry-MS2x4 (export tag consisting of 4 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-PH

Plasmid

#96948

Purpose

EGFP-PLC pleckstrin homology domain for CALI validation

Depositor

Marcel Bruchez

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

Pleckstrin homology domain

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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miniCMV-iRFP670-tDeg

Plasmid

#185403

Purpose

iRFP670-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

iRFP670-tDeg

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pZZMP154

Plasmid

#231760

Purpose

Mammalian expression of LZ-SPO11

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

LZ-SPO11 (Spo11 Mouse, Budding Yeast)

Tags

Flag-LeucineZipper

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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myr-palm_mCherry-mEGFP

Plasmid

#127815

Purpose

Hetero-dimer mCherry-mEGFP expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

myristoylated-palmitoylated-mCherry-mEGFP

Expression

Mammalian

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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TNXXL-CTS20

Plasmid

#48342

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

CTS20

Tags

TN-XXL

Expression

Mammalian

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
Bongwoori-R3

Plasmid

#101658

Purpose

A variant of Bongwoori with H237R mutation in its linker region. (* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

Bongwoori-R3

Expression

Mammalian

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBT140

Plasmid

#27493

Depositor

Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

Nuclear localization signal-Cre

Tags

nuclear localization signal

Expression

Mammalian

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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Rtn3c-mEmerald

Plasmid

#186963

Purpose

Express Rtn3c with C-terminus mEmerald

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

RTN3C (Rtn3 Mouse)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pVH233-Tier1-PmPGK1-eYFP

Plasmid

#169522

Purpose

Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven enhanced yellow fluorescent protein

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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TRPML2-HA

Plasmid

#18828

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pFH2.21_mCherry-MS2x6

Plasmid

#205536

Purpose

Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.11_Gag-MCP_ZF2-P2A-eGFP

Plasmid

#205526

Purpose

Express HIV Gag-MCP_ZF2-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP_ZF2

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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BLING 1

Plasmid

#171647

Purpose

Expresses bioluminescent glutamate indicator in mammalian cells

Depositor

Assaf Gilad

Article

Petersen et al ACS Synth Biol. 2023 Jul 14. doi: 10.102

Insert

BLING 1

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDD2701

Plasmid

#175078

Purpose

Mammalian expression of Flag-tagged KSHV ORF45

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8 )

Expression

Mammalian

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBBI

Plasmid

#65795

Purpose

Vector for iCre mRNA synthesis

Depositor

Masato Ohtsuka

Article

Ohtsuka et al Transgenic Res. 2013 Aug;22(4):873-5. do

Insert

iCre

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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mGarnet2-RITA

Plasmid

#104314

Purpose

codes for a fusion of fluorescent protein mGarnet2 and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cells

Depositor

Ulrich Nienhaus

Article

Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

Insert

mGarnet2-RITA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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psgRNA-ccdB

Plasmid

#99915

Purpose

Basic cloning vector for the expression of imaging-based optimization sgRNA

Depositor

Yujie Sun

Article

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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