We narrowed to 21,630 results for: ESC

1. pT2-7xTcf-NLS-ONL-CP

   Plasmid

   #65716

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2-7xTcf-NLS-YNL-CP

   Plasmid

   #65714

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDual-eGFP(W66)

   Plasmid

   #63217

   Purpose

   Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP(W66)
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Mutation

   Changed tyrosine at position 66 with respect to t…

   Promoter

   CMV-EF1α hybrid (CEF)

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDual-eGFP(Stop66)

   Plasmid

   #63218

   Purpose

   Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP(Stop66)
   

   Use

   Lentiviral

   Tags

   T7 and his6

   Expression

   Bacterial and Mammalian

   Mutation

   Changed tyrosine at position 66 with respect to t…

   Promoter

   CMV-EF1α hybrid (CEF)

   Available since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pT2-7xTcf-NLS-CNL-CP

   Plasmid

   #65715

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2-7xTcf-NLS-YNL

   Plasmid

   #65711

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, YNL
   

   Use

   Luciferase; Tol2 system

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDRF'- AmTryoshka1;3-LS-F138I

   Plasmid

   #100922

   Purpose

   Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   AmTryoshka1;3-LS-F138I (AMT1;3 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   F138I

   Promoter

   PMA

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRF'- AmTryoshka1;3-LS-L255I

   Plasmid

   #100964

   Purpose

   Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   AmTryoshka1;3-LS-L255I (AMT1;3 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   L255I

   Promoter

   PMA

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDRF'- AmTryoshka1;3-GS-F138I

   Plasmid

   #100965

   Purpose

   Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   AmTryoshka1;3-GS-F138I (AMT1;3 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   F138I

   Promoter

   PMA

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pDRF'- AmTryoshka1;3-GS-L255I

    Plasmid

    #100966

    Purpose

    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-GS-L255I (AMT1;3 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L255I

    Promoter

    PMA

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid

    #225139

    Purpose

    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

    Depositor

    Urartu Seker

    Article

    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.

    Insert

    Ag43 protein fused to silaffin-R5 and sfGFP
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-PRKCZ-C1 (N-PRKCZ-C1)

    Plasmid

    #217760

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C1 domain (aa 123-193)

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pBactin-AcGFP-CI

    Plasmid

    #196849

    Purpose

    Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBICI_high ori

    Plasmid

    #179273

    Purpose

    Expresses CuCBP and BaSP in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lacO

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDUBI

    Plasmid

    #179274

    Purpose

    Expresses CcCDP monocistronically and CuCBP and BaSP bicistronically

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    cellodextrin phosphorylase
    cellobiose phosphorylase
    C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
    

    Use

    Synthetic Biology

    Tags

    6xHIS and Strep-Tag II

    Expression

    Bacterial

    Mutation

    sucrose phosphorylase

    Promoter

    T7 lacO

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. iDianiSnFR

    Plasmid

    #177741

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    iDianiSnFR
    

    Use

    Tags

    HA, His, and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. iCytSnFR

    Plasmid

    #177738

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    iCytSnFR
    

    Use

    Tags

    HA, His, and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. iCyt_BrEt_SnFR

    Plasmid

    #177740

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    iCyt_BrEt_SnFR
    

    Use

    Tags

    HA, His, and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. iCyt_F_SnFR

    Plasmid

    #177739

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    iCyt_F_SnFR
    

    Use

    Tags

    HA, His, and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJOG252

    Plasmid

    #80584

    Purpose

    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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