We narrowed to 75,832 results for: Rest

1. lenti-Cas9-VQR-GFP_activity_reporter

   Plasmid

   #87156

   Purpose

   This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

   Depositor

   Daniel Bauer

   Article

   Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

   Insert

   GFP and sgRNA targeting GFP (NGA restricted)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. RCAS-sgATG7

   Plasmid

   #75228

   Purpose

   An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

   Depositor

   Noor Gammoh

   Article

   Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

   Insert

   Autophagy Related 7 (Atg7 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pWzl_neo_DEST_flag_SOX2

   Plasmid

   #45309

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   MCOPS3 (SOX2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

4. AAVS1 intron 1 gRNA 27

   Plasmid

   #132396

   Purpose

   Targets AAVS1 intron 1, gRNA: ACCCCACAGTGGGGCCACTA, MLM3636 backbone

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSP1-mp53AS-6xMUT

   Plasmid

   #20904

   Depositor

   Stefano Piccolo

   Article

   Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

   Insert

   p53AS (Trp53 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   point mutation (Ser/Thr-Ala) of the 6 N-terminal …

   Promoter

   Available since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

6. PC1575

   Plasmid

   #232088

   Purpose

   Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.

   Depositor

   Pranam Chatterjee

   Article

   Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi

   Insert

   uAb with cloning Esp3I restriction site
   

   Use

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-sgTnr#2/Cre

   Plasmid

   #193245

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tnr gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgTnr#2 (Tnr Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-sgTnr#1/Cre

   Plasmid

   #193244

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tnr gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgTnr#1 (Tnr Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pODN-H2BC11-TagBFP

   Plasmid

   #183867

   Purpose

   Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mTagBFP

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PEA1-Nuclease

    Plasmid

    #176528

    Purpose

    Delivers all prime editing nuclease components in a single plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    GC to CA point mutation in SpCas9 to restore nucl…

    Promoter

    CbH for Cas9, hU6 for gRNAs

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1 intron 1 gRNA s3

    Plasmid

    #132395

    Purpose

    Targets AAVS1 intron 1, gRNA: GGGGCCACTAGGGACAGGAT, MLM3636 backbone

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3/ TO myc-cCE-bio (cCE-bio)

    Plasmid

    #82473

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive form

    Depositor

    Daniel Schoenberg

    Article

    Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

    Insert

    Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Synthetic, Mouse)
    

    Use

    Tags

    TEV-Biotin and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)

    Plasmid

    #50866

    Purpose

    Expresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C I730C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 Flag YFP hNKCC1 I674C (NT475)

    Plasmid

    #50875

    Purpose

    Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    I674C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 Flag YFP hNKCC1 N680C A734C (NT816)

    Plasmid

    #50870

    Purpose

    Expresses human NKCC1 N680C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    N680C A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 Flag YFP hNKCC1 L737C (NT523)

    Plasmid

    #50891

    Purpose

    Expresses human NKCC1 L737C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    L737C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 Flag YFP hNKCC1 P676C A734C (NT810)

    Plasmid

    #50867

    Purpose

    Expresses human NKCC1 P676C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 Flag YFP hNKCC1 N672C (NT474)

    Plasmid

    #50873

    Purpose

    Expresses human NKCC1 N672C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    N672C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 Flag YFP hNKCC1 P676C (NT443)

    Plasmid

    #50877

    Purpose

    Expresses human NKCC1 P676C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 Flag YFP hNKCC1 N731C (NT505)

    Plasmid

    #50885

    Purpose

    Expresses human NKCC1 N731C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    N731C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge