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MT4

Plasmid

#223332

Purpose

Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)

Tags

3HA-miniTurboID-EGFP

Expression

Plant

Promoter

CaMV35S

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mStayGold-EZR

Plasmid

#227294

Purpose

Donor template for mStayGold insertion into the C-terminus of the EZR locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-EZR (Addgene #227293)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

EZR Homology Arms flanking a mStayGold Tag (EZR Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pME-myr-mTagBFP2-Flag (JDW 1183)

Plasmid

#224492

Purpose

Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

myr-mTagBFP2-Flag

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA1_Cas9-hGem_Smc4

Plasmid

#217667

Purpose

expresses Cas9-hGem and guideRNA for tagging of SMC4

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA1 targeting SMC4 C-terminal (SMC4 Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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Hygro-P2A-HA-FKBP-WAPL

Plasmid

#217666

Purpose

Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

WapI (WAPL Human)

Tags

dTag (FKBP36V protein)

Expression

Bacterial and Mammalian

Promoter

Lac

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-L819Y-siRNAres_AC

Plasmid

#148394

Purpose

Insect Expression of DmAGO1-L819Y-siRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmAGO1-L819Y-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-P714GA744FL819Y-siRNAres_AC

Plasmid

#148395

Purpose

Insect Expression of DmAGO1-P714GA744FL819Y-siRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmAGO1-P714GA744FL819Y-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 silent mutations compared to the…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNAres_AC

Plasmid

#148404

Purpose

Insect Expression of DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot1iso1_1318-1605-shRNAres_AC

Plasmid

#148414

Purpose

Mammalian Expression of HsNot1iso1_1318-1605-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsNot1iso1_1318-1605-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A_1-1609-del1384-1524-siRNAres_N

Plasmid

#147024

Purpose

Mammalian Expression of HsTNRC6A_1-1609-del1384-1542-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6A_1-1609-del1384-1542-siRNAres (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-siRNAres-HA_O

Plasmid

#147097

Purpose

Insect Expression of DmPABPC1-siRNAres

Depositor

Elisa Izaurralde

Article

Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

Insert

DmPABPC1-siRNAres (pAbp Fly)

Expression

Insect

Mutation

seven silent mutations compared to the sequence g…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1_1-550-siRNAres_L

Plasmid

#146859

Purpose

Insect Expression of DmPABPC1_1-550-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_1-550-siRNAres (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and six silent muta…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1_90-635-siRNAres-V5His6_L

Plasmid

#146860

Purpose

Insect Expression of DmPABPC1_90-635-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_90-635-siRNAres (pAbp Fly)

Expression

Insect

Mutation

five silent mutations compared to the sequence gi…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del91-164-V5His6_L

Plasmid

#146861

Purpose

Insect Expression of DmPABPC1_del91-164

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del91-164 (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and five silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182_1-1199-del862-1115-dsRNAres_L

Plasmid

#146820

Purpose

Insect Expression of DmGW182_1-1199-del862-1115-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182_1-1199-del862-1115-dsRNAres (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-del1352-1369-siRNAres_K

Plasmid

#146743

Purpose

Mammalian Expression of HsTNRC6A-del1352-1369-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6A-del1352-1369-siRNAres (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-3 lentiCRISPR v2-Blast plasmid

Plasmid

#192233

Purpose

lentiviral vector expressing sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid

Plasmid

#192234

Purpose

lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-2 lentiCRISPR v2 plasmid

Plasmid

#192232

Purpose

lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-1 lentiCRISPR v2 plasmid

Plasmid

#192231

Purpose

lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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