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We narrowed to 6,447 results for: nls

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Showing: 3061 - 3080 of 6447 results

  1. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

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  3. pSFV_Nb139-NLS-VP64 (3'UTR)_RLEloop

    Plasmid
    #240256
    Purpose
    Attenuated PROTEUS vector with Nb139-VP64 transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.
    Depositor
    Gregory Neely
    Article
    Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:
    Insert
    nanobody Nb139
    Tags
    NLS-VP64
    Expression
    Mammalian
    Mutation
    VP64 = 4*VP16[aa437-447]
    Promoter
    SFV subgenomic promoter
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    240256-plasmid-map-sequence-id-487183

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  7. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

    Plasmid
    #221537
    Purpose
    Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221537-plasmid-map-sequence-id-437448

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  8. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid
    #221540
    Purpose
    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221540-plasmid-map-sequence-id-437823

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  20. CAGGS-safb∆sap-NLS-3XFLAG-V5

    Plasmid
    #196091
    Purpose
    Expresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 31-65 (SAP domain) of mouse S…
    Promoter
    CAGGS
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196091-plasmid-map-sequence-id-384675

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Showing: 3061 - 3080 of 6447 results