160,922 results

1. pCMV-PE6a

   Plasmid

   #207851

   Purpose

   Mammalian expression of PE6a prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6a
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29b-H6_Streptavidin_sfGFP

   Plasmid

   #124296

   Purpose

   His6-tagged Streptavidin GFP fusion protein

   Depositor

   Mark Arbing

   Insert

   His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
   

   Use

   Tags

   His6 and T7 tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Artichoke

   Plasmid

   #73320

   Purpose

   Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

   Depositor

   Benjamin Ebert

   Article

   Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and EF1a

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcdna nesprin TS

   Plasmid

   #68127

   Purpose

   nesprin tension sensor

   Depositor

   Daniel Conway

   Article

   Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

   Insert

   mouse nesprin mini 2G (Syne2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Syn-DIO-PinkyCaMP

   Plasmid

   #232857

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under Syn promoter in a Cre-dependent manner (DIO, double floxed inverted open reading frame)

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

   Plasmid

   #197502

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBOBI-FLuc

   Plasmid

   #170674

   Purpose

   Express firefly luciferase. Used in lentivirus-based SARS-CoV-2 related (MERS) pseudovirus assay.

   Depositor

   David Nemazee

   Article

   Rogers et al Science. 2020 Aug 21;369(6506):956-963.

   Insert

   luc
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. phND2-N174

   Plasmid

   #31822

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

   Insert

   NeuroD2 (NEUROD2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-Flp

   Plasmid

   #122969

   Purpose

   Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

   Depositor

   Lydia Freddolino

   Article

   Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

   Insert

   Flp recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Aug. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64

    Plasmid

    #203348

    Purpose

    Template for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

    Depositor

    Christopher Grigsby, Molly Stevens

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Template for t7-mediated in vitro transcription o…

    Tags

    Flanked by 5' and 3' UTRs from Xenopus …

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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