We narrowed to 7,778 results for: CCH
-
Plasmid#38227DepositorInsertHIS3 (HIS3 Synthetic, Budding Yeast)
TagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3Available SinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH737-CEN-maxHIS3
Plasmid#38229DepositorInsertHIS3 (HIS3 Synthetic, Budding Yeast)
TagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3 (=GPD)Available SinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pILGFP84
Plasmid#83542PurposeUsed to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.DepositorInsertSSA1 promoter-yEGFP
ExpressionYeastPromoterSSA1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RLuc8C-FHA1-NES
Plasmid#138210PurposeEncodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.DepositorInsertRLuc8C-FHA1-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
A102 ΔAmpH Δ2x+ve
Plasmid#129484PurposeExpression of Glo3 ΔAmpH lacking the COPI-binding motif in E.ColiDepositorInsertADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
TagsHis and MBPExpressionBacterialAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKV005
Plasmid#64763PurposeRecombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable Myc tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassettePromoternoneAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
BAS291 C terminal mKO2 kanR
Plasmid#74086PurposeS.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 resistance.DepositorInsertmKO2
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKP4010
Plasmid#113336PurposeBeta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoterDepositorInsertsArtificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase
Tags3X-FLAG/8X-Gly linkerExpressionYeastAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation
Plasmid#58830PurposeExpresses GFP-Tagged and mutated Osh2 PH domain in yeastDepositorInsert2 x Osh2 PH domain (OSH2 Budding Yeast)
TagsGFP and MycExpressionYeastMutationchanged Lysine 307 to Glutamate and Arginine 309 …PromoterPHO5Available SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGS1011
Plasmid#63783PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection markerDepositorInsertStrep-tag II followed by TRP1 selection marker
UseE. coli cloning vectorTagsStrep-tag IIPromoternoneAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
BAS290 C terminal E2C natR
Plasmid#74085PurposeS.pombe recoded E2Crimson in universal C-terminal tagging plasmid, nourseothricin resistanceDepositorInsertE2Crimson
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS313-PRP8
Plasmid#90964Purposeyeast vectorDepositorAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-Purg1-GST
Plasmid#39352DepositorUseYeast genomic targetingTagsGST and kanMX6 module - reverse orientationAvailable SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis (1-440)
Plasmid#60391PurposeExpression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2801
Plasmid#63656PurposepCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
-
pET151-Vta1(280-330) S278C
Plasmid#87739PurposeBacterial expression of a VSL domain variant of S. cerevisiae Vta1DepositorInsertVacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
Tags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-277, mutated Ser 278 to CysPromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K651R
Plasmid#84528PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2991
Plasmid#63643PurposepCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKV006
Plasmid#64764PurposeRecombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable V5 tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassettePromoternoneAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(81-437)
Plasmid#87734PurposeBacterial expression of N-terminally truncated S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-80PromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pST39-His/Vps27(345-440) - Hse(275-375)
Plasmid#17638DepositorAvailable SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB2a Cterminal tail
Plasmid#60396PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP (F64L, S65T, V163A)-His3MX6
Plasmid#53185Purposechromosomal tagging with stable GFPDepositorAvailable SinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pJH124
Plasmid#48259PurposeTemplate plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
Ag TEF1 promoter
UseRoutine cloning vectorMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterAg TEF1 promoterAvailable SinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEB0_11_5xQUAS_CYC1cp
Plasmid#236215PurposePlasmid encoding 5xQUAS_CYC1cp (five repeats of the binding sequence of QF directly upstream of the CYC1 core promoter) as a Type 2 part to be used in the Dueber Yeast MoClo Toolkit.DepositorInsertCYC1 core promoter with 5x QUAS binding sites
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEB2_15_Q-system
Plasmid#236221PurposeQ-system: Plasmid with QS under HHF2p, QF2 under PGK1p and mTurquoise2 under 5xQUAS_CYC1cp with homologous regions for genome integration into HO locus.DepositorInsertsQS, ENO2 terminator
QF2, ACT1 terminator
mTurquoise2, ADH1 terminator
UseSynthetic BiologyExpressionBacterialPromoter5xQUAS_CYC1cp, HHF2p, and PGK1pAvailable SinceMarch 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEB2_14_Q-system-QF2-ctrl
Plasmid#236220PurposeControl for Q-system in pEB2_15: Plasmid with QF2 under PGK1p and mTurquoise2 under 5xQUAS_CYC1cp with homologous regions for genome integration into HO locus.DepositorInsertsQF2, ACT1 terminator
mTurquoise2, ADH1 terminator
UseSynthetic BiologyExpressionBacterialPromoter5xQUAS_CYC1cp and PGK1pAvailable SinceMarch 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pVG173
Plasmid#251208PurposeExpresses a version of light-inducible transcription factor EL222 (Gly80Arg) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseSynthetic BiologyExpressionBacterial and YeastMutationGly80ArgPromoterPGK1prAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-PHO8 K(24,25,30,33)R
Plasmid#207044PurposeExpression of GFP-Pho8 point mutant under CUP1 promoter.DepositorInsertPHO8
TagsGFPExpressionYeastMutationK(24,25,30,33)RPromoterpCUP1Available SinceFeb. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
UbSYRAP_pDONR201
Plasmid#249816PurposeEncodes UbSYRAP, a SYRAP split version (UBQ-RAP2.12-GAL4STE12), Gateway compatible as Donor plasmidDepositorInsertUBQ-RAP2.12-GAL4STE (RAP2.12 Budding Yeast, Mustard Weed)
UseSynthetic BiologyTagsGAL4-STE12 and UBQExpressionPlant and YeastAvailable SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
pJTB72
Plasmid#248908PurposeExpresses yeast Smk1in bacteriaDepositorInsertSMK1 Mitogen-activated Protein Kinase (SMK1 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes)
ExpressionBacterialPromoterT7Available SinceJan. 16, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYO816
Plasmid#242482PurposeExpression of Scatg38 Δ(123 to 226)-2xEGFP under its own promoterDepositorAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
TtEnd_E448A_pEHISTEV
Plasmid#179733PurposeTtEnd_E448A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which Glu448 has been mutated to Ala448.DepositorInsertTtEnd5A
Tags6xHisExpressionBacterialMutationGlu448 has been mutated to Ala448.PromoterT7Available SinceOct. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
TtEnd_E329A_pEHISTEV
Plasmid#179734PurposeTtEnd_E329A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which Glu329 has been mutated with Ala329DepositorInsertTtEnd5A
Tags6xHisExpressionBacterialMutationGlu329 has been mutated to Ala329.PromoterT7Available SinceOct. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
TtEnd_H280A_pEHISTEV
Plasmid#179735PurposeTtEnd_H280A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which His280 has been mutated with Ala280.DepositorInsertTtEnd5A
Tags6xHisExpressionBacterialMutationHis280 has been mutated to Ala280.PromoterT7Available SinceOct. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
TtEnd_CDC_pEHISTEV
Plasmid#179736PurposeTtEnd_CDC_pEHISTEV expresses tuncated variant of TtEnd5A (186-531 amni caids) comprsisng of cataytic domain and a linker at C-terminal.DepositorInsertTtEnd5A
Tags6xHisExpressionBacterialMutation185 Amino acids from N-terminal were deleted.PromoterT7Available SinceOct. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
TtEnd_CDC_E329A
Plasmid#179737PurposeTtEnd_CDC_E329A expresses TtEnd_CDC_pEHISTEV, in which Glu329 has been mutated with Ala329.DepositorInsertTtEnd5A
Tags6xHisExpressionBacterialMutationGlu329 has been mutated with Ala329PromoterT7Available SinceOct. 14, 2025AvailabilityAcademic Institutions and Nonprofits only