We narrowed to 9,302 results for: BLI;
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Plasmid#217674PurposeFor expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experimentDepositorInsertCRYGS (CRYGS Human)
Tags6XHis and SUMOExpressionBacterialMutationGlutamine 16 changed to CysteinePromoterT7Available SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-mRFP
Plasmid#218709PurposeEncodes codon optimized mRFP enzyme for bacterial expressionDepositorInsertmRFP
Tags10xHisExpressionBacterialAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-LCC-A2
Plasmid#218691PurposeEncodes codon optimized LCC-A2 enzyme for bacterial expressionDepositorInsertLCC-A2
Tags10xHisExpressionBacterialAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-IsPETase
Plasmid#218697PurposeEncodes codon optimized IsPETase enzyme for bacterial expressionDepositorInsertIsPETase
Tags10xHisExpressionBacterialAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-PES-H1
Plasmid#218704PurposeEncodes codon optimized PES-H1 enzyme for bacterial expressionDepositorInsertPES-H1
Tags10xHisExpressionBacterialAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV-hEIF5B-587~1220
Plasmid#208637Purposefor E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220DepositorInserthuman eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
Tags6xhisExpressionBacterialMutationcontain SNP rs1376443, protein sequence not chang…PromoterT7Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV/CMV-SCLM
Plasmid#216758PurposeAAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleonDepositorInsertSuperClomeleon followed by WPRE and human growth hormone polyA terminator
UseAAVExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterCMV (cytomegalovirus)Available SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
MECP2 - S411R in pENTR
Plasmid#216603Purposegateway cloning donor vector containing MECP2 ASD mutationDepositorAvailable SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
MECP2 - P388S in pENTR
Plasmid#216602Purposegateway cloning donor vector containing MECP2 ASD mutationDepositorAvailable SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
MECP2 - P376S in pENTR
Plasmid#216601Purposegateway cloning donor vector containing MECP2 ASD mutationDepositorAvailable SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
MECP2 - E282G in pENTR
Plasmid#216600Purposegateway cloning donor vector containing MECP2 ASD mutationDepositorAvailable SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
MECP2 - P217L in pENTR
Plasmid#216599Purposegateway cloning donor vector containing MECP2 ASD mutationDepositorAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
MECP2 - A202G in pENTR
Plasmid#216598Purposegateway cloning donor vector containing MECP2 ASD mutationDepositorAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
MECP2 - G195S in pENTR
Plasmid#216597Purposegateway cloning donor vector containing MECP2 ASD mutationDepositorAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
MECP2 - P152L in pENTR
Plasmid#216596Purposegateway cloning donor vector containing MECP2 ASD mutationDepositorAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
MECP2 - P152R in pENTR
Plasmid#216595Purposegateway cloning donor vector containing MECP2 ASD mutationDepositorAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
MECP2 - R91W in pENTR
Plasmid#216593Purposegateway cloning donor vector containing MECP2 ASD mutationDepositorAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-hHNF4aORF-1
Plasmid#206959PurposeThe ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.DepositorInsertHNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)
ExpressionBacterialPromoterT7Available SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-191~307-mEGFP(A206K)-TwStrep
Plasmid#208626Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 191~307, other residues not includedPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-299~398-mEGFP(A206K)-TwStrep
Plasmid#208627Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 299~398, other residues not includedPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-212~285-mEGFP(A206K)-TwStrep
Plasmid#208629Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 212~285, other residues not includedPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-62
Plasmid#208647PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
UseCRISPRExpressionMammalianPromoterCMV and U6 in tandemAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-56
Plasmid#208979PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)
UseCRISPRExpressionMammalianPromoterCMV and U6 in tandemAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-OH
Plasmid#138956PurposeUsed to generate a transgene that will express an epitope tagged Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv with C terminal epitope tag (kkv Fly)
Tagsollas and his-6 added to C terminus of protein co…ExpressionInsectPromoterUASAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-DsRed-kkv-3::NG
Plasmid#138960PurposeUsed to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.DepositorArticleInsert3' end of kkv and neon green (kkv Fly)
UseAn editing vector for drosophilaTagsNeon Green fused to C terminus of KkvAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLTR-SFFV-PTPN2 transcript variant 2-WPRE-UbC-Emerald
Plasmid#201187PurposeLentiviral vector plasmid expressing human PTPN2 transcript variant 2 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorAvailable SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLTR-SFFV-PTPN2 transcript variant 3-WPRE-UbC-Emerald
Plasmid#201188PurposeLentiviral vector plasmid expressing human PTPN2 transcript variant 3 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorAvailable SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1067L
Plasmid#200253PurposepBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomatoDepositorInsert6 gRNAs targeting Doublesex, Intersex, bTublin
ExpressionInsectAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK4-Y801H Ankyrin Repeats pET151
Plasmid#164058PurposeExpresses mouse KANK4 Ank domain -Y801H mutant in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)
TagsHis-tag, TEV cleavage siteExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOD_004
Plasmid#155361PurposeGentamycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with SalmonellaDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOD_003
Plasmid#155362PurposeZeomycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with SalmonellaDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCEP4_HA-Halo-cmyc-GRM3 C127A
Plasmid#140458PurposeMammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127ADepositorInsertGRM3 (GRM3 Human)
TagsHalo, Signal/leader sequence from influenza hemag…ExpressionMammalianMutationCysteine 127 to alanine, Threonine 174 to alanineAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44I-c014
Plasmid#175451PurposeProtein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220. Can be biotinylated.DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44U-c011
Plasmid#175454PurposeProtein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44I-c011
Plasmid#175449PurposeProtein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.DepositorInsertCD44 (CD44 Human)
ExpressionBacterialAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlY282F
Plasmid#172468Purposeexpresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domainDepositorInsertwbp2nlY282F
UseXenopus expressionTagsnoneMutationchanged Tyrosine at aa282 to PhenylalaninePromoterSP6Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlF127P
Plasmid#174507Purposeexpresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domainDepositorInsertwbp2nlF127P
UseXenopus expressionTagsnoneMutationchanged Phenylalanine at aa127 to ProlinePromoterSP6Available SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlT45A
Plasmid#172464Purposeexpresses protein with one aa substitution to affect potential phosphorylationDepositorInsertwbp2nlT45A
UseXenopus expressionTagsnoneMutationchanged Threonine at aa45 to AlaninePromoterSP6Available SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlY55F
Plasmid#172466Purposeexpresses protein with one aa substitution to affect potential phosphorylation in ITSM domainDepositorInsertwbp2nlY55F
UseXenopus expressionTagsnoneMutationchanged Tyrosine at aa55 to PhenylalaninePromoterSP6Available SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB6-Id1-T2A-sfGFP-iCre-ERT2-FNF-TK
Plasmid#139279PurposeB6J Id1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy numberDepositorInsertId1 (Id1 Mouse)
UseCre/Lox and Mouse TargetingTagsC-terminal fusion of T2A-sfGFP-iCre-ERT2ExpressionMammalianAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFE-sfGFP
Plasmid#162688PurposepFE vector control expressing sfGFPDepositorInsertsfGFP
TagsC terminal 6x His tagExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFP.R373
Plasmid#138243PurposeExpresses the split mCherry reporter interrupted by the SaP-dpol intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R362
Plasmid#138232PurposeExpresses the split mCherry reporter interrupted by the CbP-RNR artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R376
Plasmid#138246PurposeExpresses the split mCherry reporter interrupted by the Pfu-RIR1-1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R363
Plasmid#138233PurposeExpresses the split mCherry reporter interrupted by the Ckl-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R366
Plasmid#138236PurposeExpresses the split mCherry reporter interrupted by the LLP-Pol intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R367
Plasmid#138237PurposeExpresses the split mCherry reporter interrupted by the LP-Hel intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R368
Plasmid#138238PurposeExpresses the split mCherry reporter interrupted by the MP-B-DnaB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R369
Plasmid#138239PurposeExpresses the split mCherry reporter interrupted by the MP-K-gp53 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R370
Plasmid#138240PurposeExpresses the split mCherry reporter interrupted by the MP-C-gp206 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only