We narrowed to 16,140 results for: IGH@

1. pET-NCBD + ACTR

   Plasmid

   #99335

   Purpose

   Bacterial coexpression of CBP NCBD and NCoA3 binding domains

   Depositor

   Peter Wright

   Article

   Demarest et al Nature. 2002 Jan 31;415(6871):549-53.

   Inserts

   CBP NCBD (2059-2117) (CREBBP Mouse)
   SRC3 (1023-1093) (NCOA3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MET-bio-His

   Plasmid

   #52022

   Purpose

   Expresses full-length Hepatocyte growth factor receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   MET (MET Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PLUNC-bio-His

   Plasmid

   #53428

   Purpose

   Expresses full-length Palate lung and nasal epithelium clone protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PLUNC (BPIFA1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)

   Plasmid

   #99343

   Purpose

   Bacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TAD

   Depositor

   Peter Wright

   Article

   Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

   Inserts

   Hif1a (776-826) (HIF1A Human)
   CBP (340-439)
   

   Use

   Tags

   His6GB1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6-hGRIN2B-CAG-ps-SaCas9

   Plasmid

   #102853

   Purpose

   A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B Human, S. aureus, Synthetic)
   

   Use

   CRISPR

   Tags

   3X Flag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter;

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. LAMP1-bio-His

   Plasmid

   #51809

   Purpose

   Expresses full-length Lysosome-associated membrane glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A

   Plasmid

   #74606

   Purpose

   expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   full length human dynein light intermediate chain 1 Y101A, Y103A, W120A (DYNC1LI1 Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Y101A, Y103A, W120A

   Promoter

   SFFV

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-mCherry-p2A-LIC1 Y101A, Y103A

   Plasmid

   #74605

   Purpose

   expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   full length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Y101A, Y103A

   Promoter

   SFFV

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX Btk-SH2

   Plasmid

   #46412

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Btk (BTK Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 271-385

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. LRRC-bio-His

    Plasmid

    #53333

    Purpose

    Expresses full-length Leucine-rich repeat-containing protein 32 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    LRRC (LRRC32 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EBL1-bio

    Plasmid

    #47792

    Purpose

    Expresses enzymatically monobiotinylated full-length EBL1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised EBL1
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)

    Plasmid

    #99342

    Purpose

    Bacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TAD

    Depositor

    Peter Wright

    Article

    Wojciak et al EMBO J. 2009 Apr 8;28(7):948-58. doi: 10

    Inserts

    STAT1 (710-750) (STAT1 Human)
    CBP (1764-1855)
    

    Use

    Tags

    His6GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6P1-human LIC1 C-terminal half

    Plasmid

    #74599

    Purpose

    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    

    Use

    Tags

    N-terminal GST and Strep II

    Expression

    Bacterial

    Mutation

    amino acids 389-523 of LIC1

    Promoter

    T7

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. DLK1-bio-His

    Plasmid

    #51876

    Purpose

    Expresses full-length Delta-like protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    DLK1 (DLK1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ITGA2B-bio-His

    Plasmid

    #53353

    Purpose

    Expresses full-length Integrin alpha-IIb precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ITGA2B (ITGA2B Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GP1BA-bio-His

    Plasmid

    #51907

    Purpose

    Expresses full-length Platelet glycoprotein Ib alpha chain precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    GP1BA (GP1BA Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV2_hSyn_iChloC_CA_Citrine

    Plasmid

    #98220

    Purpose

    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iChloC_C128A gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    E83Q, E90R, E101S, C128A, T159C, D156N

    Promoter

    human synapsin

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. rd-his

    Plasmid

    #112911

    Purpose

    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag and non-cleavable C-terminal 6-his tag

    Depositor

    C. James McKnight

    Article

    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20

    Insert

    dematin (DMTN Human)
    

    Use

    Tags

    6-his tag, Glutathione-S-Transferase, and preciss…

    Expression

    Bacterial

    Mutation

    89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAG…

    Promoter

    T7

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCIBN-tagBFP-hNCL

    Plasmid

    #103805

    Purpose

    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hNCL (CIB1, NCL Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCIBN-LacI

    Plasmid

    #103814

    Purpose

    BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-LacI (CIB1, lacI E. coli, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    LacI: C19T (silent, L7L), T264C (silent, A88A), C…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge