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Addgene

We narrowed to 7,157 results for: Tet-on

Showing: 3081 - 3100 of 7157 results
  1. p2B-55

    Plasmid
    #177924
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Depositor
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    TDH3
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pTE5427

    Plasmid
    #229034
    Purpose
    tetO-phi29 DNA polymerase ‘dead’ reporter: PT7-tetO-B0035-p2 D249E-T7 terminator, AmpR
    Depositor
    Insert
    PT7-tetO-RBS35-phi29 DNAP (D249E, dead)-T7term
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7-tetO
    Available since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pTE5451

    Plasmid
    #231066
    Purpose
    tetO-10xHis-phi29 DNA polymerase PovSak reporter: PT7-tetO-B0035-10xHis-p2 povsak-T7 terminator, AmpR
    Depositor
    Insert
    PT7-tetO-B0035-10xHis-phi29 DNAP PovSak-T7term
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7-tetO
    Available since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pMJH65

    Plasmid
    #67273
    Purpose
    Conjugally transferable red recombinogenic plasmid
    Depositor
    Insert
    tetA
    Use
    Red recombinase, temperature sensitive
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tetA promoter of plasmid pACYC184 origin
    Available since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Insert
    F5-flanked tetracycline resistance cassette
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Promoter
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pTE5452

    Plasmid
    #231067
    Purpose
    Inactive tetO-10xHis-phi29 DNA polymerase wild-type reporter: PT7-tetO-B0035-10xHis-p2 dead-T7 terminator, AmpR
    Depositor
    Insert
    PT7-tetO-B0035-10xHis-phi29 DNAP dead-T7term
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7-tetO
    Available since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pMS05

    Plasmid
    #230069
    Purpose
    Inducible Promoter (anhydrotetracycline-induced, tetO/tetR system), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    anhydrotetracycline-inducible
    Available since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pMS04

    Plasmid
    #230068
    Purpose
    Inducible Promoter (anhydrotetracycline-induced, tetO/tetR system), integrates via IntN1; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    anhydrotetracycline-inducible
    Available since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pCL27-decoder

    Plasmid
    #159449
    Purpose
    Insert: Pfus1tet-citrine-tCyc1. The fluorescent protein Citrine is inducible by alpha-factor and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.
    Depositor
    Insert
    Pfus1tet-citrine-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pfus1tet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pET-Sfp R4-4 mutant

    Plasmid
    #173491
    Purpose
    Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.
    Depositor
    Insert
    Sfp-R44
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pCL28-decoder

    Plasmid
    #159450
    Purpose
    Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.
    Depositor
    Insert
    PlexAtet-LexA-ER-B112-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PlexAtet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pS5

    Plasmid
    #231614
    Purpose
    L-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signals
    Insert
    Pbad-TetR-sfGFP-Ptet-AraC-mKate2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pbad/Ptet
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 3081 - 3100 of 7157 results