We narrowed to 8,572 results for: reporter

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PiggyBac-TetO-Lmx1a-t2a-Pitx3-puro

Plasmid

#176484

Purpose

TetO-Lmx1a-t2a-Pitx3 in a PiggyBac vector with puromycin resistance

Depositor

Marius Wernig

Article

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

Lmx1A-T2A-Pitx3 (PITX3, LMX1A Human)

Expression

Mammalian

Promoter

TetO

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSJX016

Plasmid

#232324

Purpose

Genome editing in Caulobacter crescentus

Depositor

Wei Zhao

Article

Sun et al Nucleic Acids Res. 2025 Apr 22;53(8):gka

Inserts

sgRNA
SpCas9M

Use

CRISPR

Tags

H-arms and sfGFP

Expression

Bacterial

Promoter

PJ23119 and Pvan

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FGFR3c-V5/HIS

Plasmid

#201108

Purpose

expression of human FGFR3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR3 receptor tyrosine kinase, variant c, full length, wildtype (FGFR3 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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OMM-short-CFAST10

Plasmid

#233597

Purpose

Expression of CFAST10 on the OMM membrane after a short linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

CFAST10

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MET-V5/HIS

Plasmid

#201982

Purpose

expression of human MET receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

MET receptor tyrosine kinase (MET Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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PiggyBac-TetO-En1-t2a-Foxa2-hygro

Plasmid

#176483

Purpose

TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance

Depositor

Marius Wernig

Article

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

En1-t2a-FoxA2 (FOXA2, En1 Mouse, Human)

Expression

Mammalian

Promoter

TetO

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mTagBFP2-blast

Plasmid

#125239

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mTagBFP2 (codon optimized)

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mRuby3-6xFLAG

Plasmid

#161613

Purpose

Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

mRuby3-6xFLAG

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human synapsin 1 promoter

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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IronFistMUT.

Plasmid

#243008

Purpose

Iron binding deficient IronFist

Depositor

Roland Malli

Article

Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

Inserts

hemerythrin-like-mNeonGreen (FBXL5 Human)
T2A
mCherry

Use

Gateway

Tags

mNeonGreen

Expression

Mammalian

Mutation

H15A, H57A, E58A, E61A, H126A, E130A

Promoter

human phosphoglycerate kinase (hPGK)

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCas9-Venus

Plasmid

#70267

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

Depositor

Daniel Bauer

Article

Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

Inserts

Cas9
Venus Reporter

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS and P2A

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF1-4X

Plasmid

#120282

Purpose

Produce human recombinant FGF1-4X in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Basic fibroblast growth factor 1 mutant

Tags

6x His tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLT3-D835Y-V5/HIS

Plasmid

#236007

Purpose

expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)

Tags

V5/His

Expression

Mammalian

Mutation

D835Y substitution

Promoter

CMV

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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PM-RspA-CFAST

Plasmid

#233601

Purpose

Expression of RspA-CFAST on the PM facing the cytosol

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-CFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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AICSDP-34: TNNI1-mEGFP

Plasmid

#114411

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette

Depositor

Allen Institute for Cell Science

Article

Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

Insert

TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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MYH11-NanoLuc-2A-tdTomato

Plasmid

#132569

Purpose

Donor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele

Depositor

James Thomson

Article

Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

Insert

Human MYH11 knockin homology arms (MYH11 Human)

Expression

Mammalian

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pmRFP-N1 human cofilin R21Q

Plasmid

#51279

Purpose

expresses human cofilin R21Q mutant fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

Insert

cofilin 1 (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

R21Q, non-rod-forming mutation that can be used t…

Promoter

CMV

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pSJX022

Plasmid

#232325

Purpose

Genome editing in Caulobacter crescentus

Depositor

Wei Zhao

Article

Sun et al Nucleic Acids Res. 2025 Apr 22;53(8):gka

Inserts

sgRNA
SpCas9M

Use

CRISPR

Tags

H-arms and cat

Expression

Bacterial

Promoter

PJ23119 and Pvan

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-hCD59-T2A-GFP

Plasmid

#205460

Purpose

lentiviral plasmid for expression of human CD59

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD59 (CD59 Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHL-H1-ccdB-mEF1a-RiH

Plasmid

#60601

Purpose

Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Inserts

Red Fluorescent Protein
Hygromycin resistance gene

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pHL-EF1a-SphcCas9-iP-A

Plasmid

#60599

Purpose

Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

CRISPR Cas9

Use

CRISPR

Expression

Mammalian

Mutation

Codon usage optimized for human usage

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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