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pCAG-OSF-PP-human-CHMP3(150)

Plasmid

#154181

Purpose

expresses human CHMP3(150) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (CHMP3 Human)

Tags

FLAG and Strep-Tag II

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 150

Promoter

CAG

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-NFIB-T2A-miRFP670

Plasmid

#187222

Purpose

Expresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Nuclear Factor I B (NFIB Human)

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1A-HA-Jag1

Plasmid

#46052

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Use

Entry vector

Tags

HA tag

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pLenti-xBE3-P2A-Puro

Plasmid

#110870

Purpose

Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)-BE3

Use

Lentiviral

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-cmlc2-TagBFP

Plasmid

#117780

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-HLA-B0702-His

Plasmid

#104849

Purpose

HLA-B0702 his tagged

Depositor

Sébastien Walchli

Article

Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

Insert

major histocompatibility complex, class I, B (HLA-B Human)

Use

Entry vector for gateway system

Tags

his

Promoter

none

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL1-9155

Plasmid

#115460

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL1-9155 (MBNL1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Triple ORF LoxP ifgMosaic Donor (LH208)

Plasmid

#99625

Purpose

To clone the 3 ORFs for Cre dependent generation of ifgMosaics

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pExp-RBD-CHis

Plasmid

#195002

Purpose

Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

Depositor

Marko Hyvönen

Article

Fischer et al bioRxiv 2023.01.10.523494

Insert

RBD (S SARS-CoV-2)

Tags

His8

Expression

Bacterial

Promoter

T7lac

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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LoxP1-His-H2B-Cherry-2A (SO290)

Plasmid

#99616

Purpose

To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

6xHis-H2B-Cherry

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-cmlc2-eGFP

Plasmid

#117782

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-gcry-eGFP

Plasmid

#117791

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-gcry-mRFP

Plasmid

#117778

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGL

Plasmid

#119952

Purpose

Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

gpps
limS

Expression

Bacterial

Mutation

N-terminal truncation of 56 amino acid residues (…

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-cmlc2-eGFP

Plasmid

#117776

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-gcry-TagBFP

Plasmid

#117771

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-mRFP

Plasmid

#117766

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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-53 bp human IFN-gamma luc

Plasmid

#17597

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter (IFNG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-53 to +70 of promoter

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-Dcst2-3xHA

Plasmid

#183543

Purpose

Expression vector of mouse DC-STAMP domain containing 2 (Dcst2) tagged with 3xHA at C-terminus.

Depositor

Masahito Ikawa

Article

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Insert

DC-STAMP domain containing 2 (Dcst2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-cmlc2-TagBFP

Plasmid

#117786

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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