We narrowed to 21,630 results for: ESC

1. pOTTC590 - pCMV ER-iRFP

   Plasmid

   #59136

   Purpose

   A plasmid that express endoplasmic reticulum-targeted iRFP under the CMV promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   ER-localized Infrared Fluorescent Protein
   

   Use

   Tags

   ER retention signal and signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-sfCherry11-ß-actin

   Plasmid

   #70221

   Purpose

   Expresses sfCherry11-ß-actin in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   sfCherry11-actin (ACTB Human)
   

   Use

   Tags

   sfCherry11

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRIPZ-TIR1-HA

   Plasmid

   #172610

   Purpose

   Lentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   TIR1
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Tet/ON Minimal CMV promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-miRFP-6xFLAG

   Plasmid

   #161614

   Purpose

   Cell morphology marker construct. Contains a near infrared fluorescent protein miRFP and six FLAG tags.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   miRFP-6xFLAG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human synapsin 1 promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-mRuby3-6xFLAG

   Plasmid

   #161613

   Purpose

   Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   mRuby3-6xFLAG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human synapsin 1 promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPBbsr2-Necl-5-ScNeo

   Plasmid

   #170283

   Purpose

   To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

   Insert

   a recombinant mouse Necl-5 fused to the mScarlet and to the mNeonGreen fluorescent protein (Pvr Mouse, Synthetic)
   

   Use

   Tags

   mScarlet and mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIG-602_NY-ESO-1_TCR_RFP

   Plasmid

   #207483

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   RFP, NY-ESO-1_TCR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC592 - pCMV Mito-iRFP

   Plasmid

   #59135

   Purpose

   A plasmid that express mitochondrial-targeted iRFP under the CMV promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Mitochondria-localized Infrared Fluorescent Protein
   

   Use

   Tags

   Mitochondria-targeting sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC591 - pCMV Mem-iRFP

   Plasmid

   #59134

   Purpose

   A plasmid that express membrane-targeted iRFP under the CMV promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Membrane-localized Infrared Fluorescent Protein
   

   Use

   Tags

   Palmitylation signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p70.unipA.NGFR.mCMV.PGK.mBFP2.p27K.WPRE

    Plasmid

    #228766

    Purpose

    insert a blue fluorescent protein mBFP2, a cyclin-dependent kinase inhibitor p27K

    Depositor

    Ferdinando Pucci

    Article

    Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

    Insert

    p27 kinase inhibitor (Cdkn1b Mouse)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC

    Plasmid

    #214950

    Purpose

    Bacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
    VNp-anti-Lysozyme Fab light chain amino-BiFC fusion
    

    Use

    Tags

    6xHis, Amino half of venus BIFC fragment, Carboxy…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCA528 CHMP6 145-201

    Plasmid

    #104597

    Purpose

    Expresses C-terminal 145-201 residues of CHMP6 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-11

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP6 residues 145-201 (CHMP6 Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA528 CHMP7 366-453

    Plasmid

    #104598

    Purpose

    Expresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP7 residues 366-453 (CHMP7 Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCHMP3-NS3-Blue

    Plasmid

    #223533

    Purpose

    Mammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.

    Depositor

    Winfried Weissenhorn

    Article

    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

    Insert

    CHMP3 (CHMP3 Human)
    

    Use

    Tags

    NS3 Hepatitis C protease and mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCHMP3-NS3-Green

    Plasmid

    #223527

    Purpose

    Mammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.

    Depositor

    Winfried Weissenhorn

    Article

    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

    Insert

    CHMP3 (CHMP3 Human)
    

    Use

    Tags

    NS3 Hepatitis C protease and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPRKCZ-C20-EGFP (C-PRKCZ-C20)

    Plasmid

    #217761

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C20 domain (aa 405-592)

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pNES-PRKCZ-C20-EGFP (C-NES-PRKCZ-C20)

    Plasmid

    #217763

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NES (ALQKKLEELELDEAPVAT) plus C20 domain (aa 405-…

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pEGFP-PRKCZ-C20 (N-PRKCZ-C20)

    Plasmid

    #217762

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Linker GGSSGGGA plus C20 domain (aa 405-592)

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCMV(MinDis)-iDianiSnFR_PM

    Plasmid

    #177751

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pCMV(MinDis)-iDianiSnFR_PM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV(MinDis)-iDianiSnFR_ER

    Plasmid

    #177750

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pCMV(MinDis)-iDianiSnFR_ER
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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