We narrowed to 7,122 results for: GFP expression plasmids

1. Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

   Plasmid

   #130665

   Purpose

   Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
   

   Use

   Ae. aegypti expression

   Expression

   Insect

   Promoter

   Aedes aegypti poly-ubiquitin

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-HDC-DIO-GFP-shRNA.scramble

   Plasmid

   #184331

   Purpose

   Expresses scramble shRNA for control expts. Flexed (DIO) cassette driven by hdc promoter fragment for pan neuronal expression

   Depositor

   William Wisden

   Article

   Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

   Insert

   hdc pan neuronal gene promoter, flex switch, GFP, scramble shRNA
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and RNAi

   Expression

   Mammalian

   Promoter

   histidine decarboxylase promoter fragment that g…

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJET-41-DExCon-antiGFPnanobody-mCherry-R11b

   Plasmid

   #179901

   Purpose

   Donor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   antiGFPnanobody-mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   TRE3GS

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWPT-ACC/ACC-mEGFP-IRES-mCherry

   Plasmid

   #49229

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Promoter

   EF1-short

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWPT-UCU/ACC-mEGFP-IRES-mCherry

   Plasmid

   #49232

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Promoter

   EF1-short

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWPT-CCC/ACC-mEGFP-IRES-mCherry

   Plasmid

   #49231

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Promoter

   EF1-short

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWPT-GGG/ACC-mEGFP-IRES-mCherry

   Plasmid

   #49230

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Promoter

   EF1-short

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)

   Plasmid

   #208289

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA; mutations in iSpyMac

   Promoter

   CMV and T7

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV hSyn syph-GFP-myc-BoNT/B(1-146)-iLID

   Plasmid

   #122981

   Purpose

   AAV plasmid with human synapsin promoter driving synaptophysin fused to GFP, iLID(V416I) and BoNT/B amino acids 1-146. Co-express with SSPB-BoNT(147-441, Y365A) for vPA-BoNT

   Depositor

   Matthew Kennedy

   Article

   Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

   Insert

   Syph-GFP-myc-BoNT/B(1-146)-iLID (Syp Rat, Synthetic)
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7-EGFP-C1-HsNot7-D40AE42A_L

    Plasmid

    #146890

    Purpose

    Mammalian Expression of HsNot7-D40AE42A. Please note that this plasmid does not contain the T7 promoter.

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    HsNot7-D40AE42A (CNOT7 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

    Plasmid

    #196702

    Purpose

    Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 wild type
    

    Expression

    Mammalian

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. [#GS24 -LV] Syn1-GFP-OMP25

    Plasmid

    #233566

    Purpose

    Lentiviral construct of mitochondrial localized GFP to be expressed in the donor cells under the Synapsin1 promotor

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    eGFP (eGFP Aequorea victoria, Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-U6/sgClta-sfGFP-Clta

    Plasmid

    #200261

    Purpose

    U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

    Depositor

    Justin Eyquem

    Article

    Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

    Insert

    sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
    

    Use

    AAV

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_hSyn_NES-Kinprola_PKA-mEGFP_WRPE-SV40

    Plasmid

    #233366

    Purpose

    hSyn1 driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_PKA-mEGFP
    

    Use

    AAV

    Tags

    NES and mEGFP

    Promoter

    hSyn1

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZac2.1 gfaABC1D-EGFP-Kir4.1

    Plasmid

    #52874

    Purpose

    Express EGFP-Kir4.1 fusion protein in astrocytes

    Depositor

    Baljit Khakh

    Article

    Tong et al Nat Neurosci. 2014 May;17(5):694-703. do

    Insert

    Kir4.1 (Kcnj10 Rat)
    

    Use

    AAV; Adeno-associated virus ()

    Expression

    Mammalian

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-5HRE-GFP PuroR

    Plasmid

    #128958

    Purpose

    Lentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent protein

    Depositor

    Carlos Carmona-Fontaine

    Article

    Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

    Insert

    5X HRE of VEGF (VEGFA Human)
    

    Use

    Lentiviral

    Tags

    d2EGFP

    Expression

    Mammalian

    Promoter

    minimal CMV promoter

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV_CAG_Kinprola_PKA-mEGFP-NLS3__WPRE-SV40

    Plasmid

    #233365

    Purpose

    CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for mammalian cell expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    Kinprola_PKA-mEGFP-NLS3X
    

    Use

    AAV

    Tags

    mEGFP-NLS3X

    Promoter

    CAG

    Available Since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-ArgiNLS-EGFP

    Plasmid

    #220594

    Purpose

    Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    EGFP
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-5HRE-GFP NeoR

    Plasmid

    #128960

    Purpose

    Lentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent protein

    Depositor

    Carlos Carmona-Fontaine

    Article

    Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

    Insert

    5X HRE of VEGF (VEGFA Human)
    

    Use

    Lentiviral

    Tags

    d2EGFP

    Expression

    Mammalian

    Promoter

    minimal CMV promoter

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40

    Plasmid

    #194690

    Purpose

    hSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Promoter

    hSyn1

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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