We narrowed to 24,600 results for: Nes

1. pET22b RNase A

   Plasmid

   #58903

   Purpose

   Bacterial expression of bovine pancreatic ribonuclease A

   Depositor

   Ronald Raines

   Article

   delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

   Insert

   RNase A (RNASE1 Bovine)
   

   Use

   Tags

   pelB sequence

   Expression

   Bacterial

   Mutation

   See Depositor Comments below

   Promoter

   T7

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMT-PTE2A

   Plasmid

   #111773

   Purpose

   Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Type

   Empty backbone

   Use

   Cloning intermediate

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB1_alpha1

   Plasmid

   #68224

   Purpose

   GoldenBraid level 1 Alpha plant expression vector.

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)/Puro/TagRFP-LL5b

   Plasmid

   #112828

   Purpose

   transient/stable overexpression

   Depositor

   Joshua Sanes, Tomasz Prószyński

   Article

   Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

   Insert

   LL5b (Phldb2 Mouse)
   

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMaCTag-05

   Plasmid

   #119984

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   eGFP
   

   Use

   Pcr template

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-luxOFP

   Plasmid

   #229950

   Purpose

   Mammalian expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

   Depositor

   Andy Yeh

   Article

   Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

   Insert

   luxOFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMX/cyclinB1 (17)

   Plasmid

   #26060

   Depositor

   Jonathon Pines

   Article

   Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

8. PEX-LV

   Plasmid

   #12120

   Depositor

   Inder Verma

   Article

   Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

   Insert

   Matrix Metalloproteinase 2 (Mmp2 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   noncatalytic fragment of MMP2 comprised of the fi…

   Promoter

   Available Since

   April 27, 2007

   Availability

   Academic Institutions and Nonprofits only

9. GeNL(Ca2+)_480/pcDNA3

   Plasmid

   #85205

   Purpose

   luminescent reporter for calcium signaling

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   GeNL(Ca2+)_480
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-EF1a-BbChT

    Plasmid

    #45186

    Depositor

    Dawen Cai, Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Has Service

    AAV9

    Insert

    pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE
    

    Use

    AAV; Adeno-associated viral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1a

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-EF1a-BbTagBY

    Plasmid

    #45185

    Depositor

    Dawen Cai, Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Has Service

    AAV9

    Insert

    pAAV-EF1a-Brainbow-invert tagBFP-EYFP-WPRE
    

    Use

    AAV; Adeno-associated viral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1a

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMaCTag-23

    Plasmid

    #120002

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with Halo

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Halo
    

    Use

    Pcr template

    Tags

    Halo

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GST HP1 alpha

    Plasmid

    #24074

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 alpha (CBX5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pV1200

    Plasmid

    #111429

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJB07

    Plasmid

    #190481

    Purpose

    Targeting plasmid for 2-plasmid C. difficile mutagenesis system

    Depositor

    Joseph Sorg

    Article

    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

    Inserts

    Upstream & Downstream pyrE deletion region
    gRNA
    

    Use

    E. coli / b. subtilis - c. difficile shuttle vect…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBabe NEO mRFP1

    Plasmid

    #98397

    Purpose

    Retroviral vector for constitutive mRFP1 expression

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    mRFP1
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-luxKate

    Plasmid

    #229951

    Purpose

    Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    luxKate
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAureus-TnCAM

    Plasmid

    #199807

    Purpose

    vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

    Depositor

    Steve Salipante

    Article

    Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

    Insert

    transposon cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330A_dCas9-1x7

    Plasmid

    #63601

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMX/cyclinB1-GFP (27)

    Plasmid

    #26061

    Depositor

    Jonathon Pines

    Article

    Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge