We narrowed to 13,996 results for: eng
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Plasmid#208377PurposeEpisomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoringDepositorTypeEmpty backboneExpressionMammalianPromoterhuman CMVAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only
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pBS-beta1,4 GT-1
Plasmid#11872DepositorInsertbeta 1,4 galactosyltransferase (B4GALT1 Human)
ExpressionBacterialAvailable SinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-Sox2-17-T2A-Klf4-E2A-Myc
Plasmid#210016Purposeepisomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for integration-free naïve resetDepositorExpressionMammalianMutationSox2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CASANOVA
Plasmid#113036PurposeAAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9DepositorInsertCASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
NG0886 pCI-TPI-WT-4H
Plasmid#65801PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
Tags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA
Plasmid#197027PurposeVector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorInserthumanized enRhCas12f1
ExpressionMammalianMutationL270RPromoterCBh, CMV, hU6Available SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
MMLV-RT(dRH) for Split-PE (pEH50)
Plasmid#190106PurposeExpresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE useDepositorInsertMMLV-RT(delta RNAseH)
TagsbpNLSExpressionMammalianMutationmutations from RT in PE2 and truncation of RNAse …PromoterCMVAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAMP-CY1_NF1 T1 (+minintr)
Plasmid#119204Purposetransfer vectorDepositorInsertNF1 type 1 mini-gene (NF1 Human)
UseTransfer vectorAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgPBRM1-1- LentiCRISPRv2
Plasmid#107403PurposeLentiviral expression of Cas9 and gRNA targeting PBRM1DepositorInsertgRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101103PurposeEncodes dCas9(C)-VP64 with SAM components fused to VEGFR1DepositorInsertVEGFR1-dCas9(C)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE
Plasmid#101104PurposeEncodes dCas9(N) fused to VEGFR2DepositorInsertVEGFR2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP
Plasmid#67299PurposeBlue light dependent photoswitching systemDepositorAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pDV002
Plasmid#191823PurposePlasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpRDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.eC
Plasmid#201903Purposenon-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.eC VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Rbfox2-rABE-Flag
Plasmid#191384PurposeDoxycycline inducible expression of Rbfox2-rABEDepositorInsertRbfox2-rABE (RBFOX2 Human, Synthetic)
UseLentiviralTagsFlagPromotertet-inducible promoterAvailable SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2 sgNRF2-4
Plasmid#186839Purposeknock out NRF2 in mammalian cellsDepositorInsertNrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
UseCRISPR and LentiviralAvailable SinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
(133) pcDNA3.1-Nup62-Flag-EPEA
Plasmid#160744PurposeExpresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymesDepositorInsertNup62-Flag-EPEA
TagsEPEA and FlagExpressionMammalianPromoterT7/CMVAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIDK-HDAC1fl
Plasmid#109158PurposeEncodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
ExpressionInsectPromoterp10Available SinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRB_LD 0_CS G_tTA-BFP
Plasmid#58876PurposeMESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only