We narrowed to 22,070 results for: ESC

1. pDUBI

   Plasmid

   #179274

   Purpose

   Expresses CcCDP monocistronically and CuCBP and BaSP bicistronically

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

   Inserts

   cellodextrin phosphorylase
   cellobiose phosphorylase
   C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
   

   Use

   Synthetic Biology

   Tags

   6xHIS and Strep-Tag II

   Expression

   Bacterial

   Mutation

   sucrose phosphorylase

   Promoter

   T7 lacO

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. iDianiSnFR

   Plasmid

   #177741

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   iDianiSnFR
   

   Use

   Tags

   HA, His, and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. iCytSnFR

   Plasmid

   #177738

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   iCytSnFR
   

   Use

   Tags

   HA, His, and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. iCyt_BrEt_SnFR

   Plasmid

   #177740

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   iCyt_BrEt_SnFR
   

   Use

   Tags

   HA, His, and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. iCyt_F_SnFR

   Plasmid

   #177739

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   iCyt_F_SnFR
   

   Use

   Tags

   HA, His, and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJOG252

   Plasmid

   #80584

   Purpose

   recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Inserts

   pnos:nptII-tnos
   p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
   pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJOG253

   Plasmid

   #80585

   Purpose

   recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Inserts

   pnos:PAT-tnos
   p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
   pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-20b-T7-Ntx-K49

   Plasmid

   #58715

   Purpose

   Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Use

   Tags

   T7 peptide tag

   Expression

   Bacterial

   Mutation

   Changed Aspartic Acid 49 to Lysine

   Promoter

   T7

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES

   Plasmid

   #138299

   Purpose

   Encodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   mCherry-PKCsub(T/A)-RLuc8N-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTUM4

    Plasmid

    #230905

    Purpose

    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

    Depositor

    Arne Skerra

    Article

    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

    Inserts

    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5HRE/GFP

    Plasmid

    #46926

    Purpose

    hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

    Depositor

    Martin Brown, Thomas Foster

    Article

    Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

    Insert

    5X HRE of VEGF (VEGFA Human)
    

    Use

    Tags

    d2EGFP

    Expression

    Mammalian

    Mutation

    five copies of a 35-bp fragment from the hypoxia-…

    Promoter

    EF-1α promoter

    Available since

    Aug. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRRLsin-SV40 T antigen-IRES-mCherry

    Plasmid

    #58993

    Purpose

    lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter

    Depositor

    Snorri Thorgeirsson

    Article

    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

    Inserts

    CMV enhancer-chicken beta actin promoter
    SV40 large T antigen
    IRES-mCherry red fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    EcoRV site in MCS converted to BamHI site

    Promoter

    CMV enhancer-chicken beta actin

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC476 - pAAV c-fos eYFP

    Plasmid

    #47907

    Purpose

    An AAV vector that expresses eYFP under the c-fos promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    enhanced Yellow Fluorescent Protein
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    c-fos

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. LiOn-CAG∞RFP

    Plasmid

    #154016

    Purpose

    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H210P

    Promoter

    CAG

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-GRAB_NE1m

    Plasmid

    #123308

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE sensor GRAB_NE1m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    contains a glycine-to-threonine mutation at posit…

    Promoter

    hSyn

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. LiOn*-CAG∞RFP

    Plasmid

    #154019

    Purpose

    Vector based on the LiOn integration-coupled translational switch and devoid of TTAA sequences, expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All TTAA in vector backbone were replaced by sile…

    Promoter

    CAG

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST pcDNA5-FRT/TO-eGFP-Rif1

    Plasmid

    #52506

    Purpose

    mammalian expression vector of Rif1 siRNA resistant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    RIF1 (RIF1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: ATAGTAC

    Promoter

    Available since

    June 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pTRIPZ-TIR1-HA

    Plasmid

    #172610

    Purpose

    Lentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    TIR1
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Tet/ON Minimal CMV promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pl-Synapsin-YFP-NaVII-III

    Plasmid

    #91246

    Purpose

    Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)

    Depositor

    Matthew Grubb

    Article

    Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.

    Insert

    Nav1.2 (Scn2a Rat)
    

    Use

    Lentiviral

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    The insert codes only for the intracellular loop …

    Promoter

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-sfCherry11x4-ß-actin

    Plasmid

    #70220

    Purpose

    Expresses sfCherry11x4-ß-actin in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    sfCherry11x4-actin (ACTB Human)
    

    Use

    Tags

    sfCherry11x4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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