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pSICO-CPSF6-358-eGFP

Plasmid

#110693

Purpose

Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFP

Depositor

Zandrea Ambrose

Article

Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

Insert

CPSF6-eGFP (CPSF6 )

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Truncation of CPSF6 isoform 2 at amino acid 358

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-S305A-3xFLAG

Plasmid

#69839

Purpose

This plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Use

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S305A

Promoter

CMV

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-SYN_mEGFP-CREB (S133A)

Plasmid

#137010

Purpose

AAV backbone, mEGFP-CREB donor for G-CREB with S133A mutation

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Use

AAV

Tags

mEGFP

Expression

Mammalian

Mutation

Changes Serine 133 into Alanine

Promoter

hSynapsin

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-GFP-miR9

Plasmid

#25036

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

mir-9-3 (MIR9-3 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-C3-PLK4-S305E-3xFLAG

Plasmid

#69840

Purpose

This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Use

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S305E

Promoter

CMV

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-GFPi

Plasmid

#31849

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 for shRNA; Ef1alpha for mCherry

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP

Plasmid

#89061

Purpose

AAV-gRNA targeting the murine Ldlr gene

Depositor

William Lagor

Article

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Chicken beta actin with partial CMV enhancer

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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1184_pAAV-U6-ApoB-gRNA2-CB-EmGFP

Plasmid

#89062

Purpose

AAV-gRNA targeting the murine Apob gene

Depositor

William Lagor

Article

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Chicken beta actin with partial CMV enhancer

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAcBac3-EcLeu TAG-EGFP**

Plasmid

#228785

Purpose

EcLeu aaRS/tRNA system for TAG suppression with EGFP containing two stop codons for dual suppression

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcLeuRS
EcLtR
EGFP**

Use

Baculoviral

Tags

Expression

Mammalian

Mutation

TAG suppressor

Promoter

Available since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231351

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CAG-EGFP-W3SL-BC(p1-10)

Plasmid

#231347

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MfnG-EcYRS-EGFP*

Plasmid

#191119

Purpose

A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein

Use

Tags

His tag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EGFP-APOBEC1-YTHmut

Plasmid

#209320

Purpose

AAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTHmut cassette.

Depositor

Magdalena Koziol

Article

Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

Insert

APOBEC1-YTHmut-HA

Use

AAV

Tags

HA

Expression

Mutation

YTH domain lacks AA 385-409 comprising the m6A-bi…

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

Plasmid

#217478

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1s

Use

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

Plasmid

#217479

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1b

Use

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-EGFP (NK350)

Plasmid

#208292

Purpose

CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-CE-ABE8e-SpCas9-BPNLS-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA inlaid into nSpCas9(D10A)

Promoter

CMV and T7

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(Luc)-GFP

Plasmid

#197887

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherry

Plasmid

#49228

Purpose

Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Lentiviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Mutation

Promoter

EF1-short

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pQLL-MAG[EF1α-copGFP]

Plasmid

#218324

Purpose

Donor plasmid of MAG Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-SB100X[EF1α-copGFP]

Plasmid

#218323

Purpose

Donor plasmid of SB100X Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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