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We narrowed to 18,050 results for: Gateway

Showing: 6281 - 6320 of 18050 results
  1. pENTR_L1-YFP-miniTurbo-NES-L2

    Plasmid
    #127351
    Purpose
    gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins
    Depositor
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

    Plasmid
    #127355
    Purpose
    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins
    Depositor
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and mVenus
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

    Plasmid
    #127356
    Purpose
    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins
    Depositor
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and mVenus
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pENTR_L1-YFP-Turbo-NES-L2

    Plasmid
    #127349
    Purpose
    gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins
    Depositor
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pECIA14

    Plasmid
    #47051
    Purpose
    This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags
    Depositor
    Type
    Empty backbone
    Use
    Secreted expression in drosophila culture
    Tags
    Alkaline Phosphatase (human, placental), Flag, HR…
    Expression
    Insect
    Mutation
    Promoter
    Metallothionein (Copper-Inducible)
    Available since
    Aug. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
  6. pECIA2

    Plasmid
    #47032
    Purpose
    This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.
    Depositor
    Type
    Empty backbone
    Use
    Secreted expression in drosophila culture
    Tags
    Fc(human IgG1), HRV 3C Protease Cleavage site, He…
    Expression
    Insect
    Mutation
    Promoter
    Metallothionein (Copper-Inducible)
    Available since
    Aug. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
  7. pYPQ230-E795L

    Plasmid
    #176890
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter
    Depositor
    Insert
    LbCas12a-E795L
    Use
    CRISPR; Gateway compatible lbcpf1 entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pENTR_L1-Turbo-NES-YFP-STOP-L2

    Plasmid
    #127358
    Purpose
    gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice
    Depositor
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pBEG R2-dsRed-L3 #BGV203

    Plasmid
    #48973
    Purpose
    Supplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    Insert
    dsRed
    Use
    Gateway cloning vector
    Tags
    IRES (strong)
    Expression
    Mutation
    Promoter
    none
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  10. pBEG R2-iBlast-L3 #BGV204

    Plasmid
    #48982
    Purpose
    Supplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    Insert
    blasticidin-S deaminase
    Use
    Gateway cloning vector
    Tags
    IRES (weak)
    Expression
    Mutation
    Promoter
    na
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  11. pBEG R2-iHygro-L3 #BGV206

    Plasmid
    #48985
    Purpose
    Supplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    Insert
    hygromycin phosphotransferase
    Use
    Gateway cloning vector
    Tags
    IRES (weak)
    Expression
    Mutation
    Promoter
    na
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  12. pENTR_L1-miniTurbo-NES-YFP-STOP-L2

    Plasmid
    #127359
    Purpose
    gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice
    Depositor
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. pYPQ290

    Plasmid
    #129670
    Purpose
    Gateway entry clone for CRISPR-Cas12b systems
    Depositor
    Insert
    AacCas12b
    Use
    CRISPR; Gateway compatible aaccas12b entry clone
    Tags
    Expression
    Plant
    Mutation
    AacCas12b is rice codon optimized
    Promoter
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. VanGlow-GL

    Plasmid
    #83342
    Purpose
    for Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    GFP::Luciferase(nls)
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
  15. pCFJ2452 - [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid
    #159854
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Insert
    [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Dec. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. pYPQ292

    Plasmid
    #129672
    Purpose
    Gateway entry clone for CRISPR-Cas12b systems
    Depositor
    Insert
    AaCas12b
    Use
    CRISPR; Gateway compatible aacas12b entry clone
    Tags
    Expression
    Plant
    Mutation
    AaCas12b is rice codon optimized
    Promoter
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 6281 - 6320 of 18050 results