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Showing: 7851 - 7900 of 24790 results

  2. XClone_Cas9_KRAB_T2A_BFP

    Plasmid
    #141208
    Purpose
    Tunable and temporal expression control of dCas9
    Depositor
    Rory Johnson
    Insert
    dCas9
    Expression
    Mammalian
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    141208-plasmid-map-sequence-id-308770

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  5. dCas9-3×RFP-P2A-CASANOVA/gRNA telo

    Plasmid
    #137711
    Purpose
    Vector co-expressing dCas9-3×RFP-P2A-CASANOVA and sgRNA telo
    Depositor
    Dominik Niopek
    Insert
    dCas9-3×RFP-P2A-CASANOVA/gRNA telo
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    137711-plasmid-map-sequence-id-273733

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  6. dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo

    Plasmid
    #137712
    Purpose
    Vector co-expressing dCas9-3×RFP-P2A-wtAcrIIA4 and sgRNA telo
    Depositor
    Dominik Niopek
    Insert
    dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    137712-plasmid-map-sequence-id-272430

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  7. pLR05_pGuide_B2M_sgRNA1

    Plasmid
    #131090
    Purpose
    sgRNA for SpCas9 mediated B2M KO
    Depositor
    Xiaojun Lian
    Insert
    B2M KO sgRNA 1 (B2M Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131090-plasmid-map-sequence-id-256909

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  8. pLR06_pGuide_B2M_sgRNA2

    Plasmid
    #131091
    Purpose
    sgRNA for SpCas9 mediated B2M KO
    Depositor
    Xiaojun Lian
    Insert
    B2M KO sgRNA 2 (B2M Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131091-plasmid-map-sequence-id-256596

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  9. pYJ12-PB-U6-ND1-acti-gRNA1

    Plasmid
    #131056
    Purpose
    Activation of NeuroD1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NeuroD1 activation gRNA1 (NEUROD1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131056-plasmid-map-sequence-id-256748

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  10. pYJ13-PB-U6-ND1-acti-gRNA2

    Plasmid
    #131057
    Purpose
    Activation of NeuroD1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NeuroD1 activation gRNA2 (NEUROD1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131057-plasmid-map-sequence-id-256893

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  11. pYJ14-PB-U6-ND1-acti-gRNA3

    Plasmid
    #131058
    Purpose
    Activation of NeuroD1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NeuroD1 activation gRNA3 (NEUROD1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131058-plasmid-map-sequence-id-256735

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  12. pRDB_246

    Plasmid
    #245333
    Purpose
    Cas12a CRISPRko positive control guide; targets CD47
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD47 (CD47 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245333-plasmid-map-sequence-id-488048

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  13. pMV_AA102

    Plasmid
    #245324
    Purpose
    Cas9 CRISPRa positive control guide; targets CD47
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD47 (CD47 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245324-plasmid-map-sequence-id-488026

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  14. 1203AE-CMV-Cas7.1-7.2-H43A-mutant

    Plasmid
    #221449
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, H to A mutation at amino acid 43 in Cas7.1
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221449-plasmid-map-sequence-id-437431

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  15. 1203AF-CMV-Cas7.1-7.2-Y55A-mutant

    Plasmid
    #221450
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, Y to A mutation at amino acid 55 in Cas7.1
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221450-plasmid-map-sequence-id-440139

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  16. 1203AG-CMV-Cas7.1-7.2-N152A-mutant

    Plasmid
    #221451
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, N to A mutation at amino acid 152 in Cas7.1
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221451-plasmid-map-sequence-id-437432

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  17. pSpec5'

    Plasmid
    #228549
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228549-plasmid-map-sequence-id-454858

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  18. pSpec3'

    Plasmid
    #228548
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228548-plasmid-map-sequence-id-454857

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  21. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160230-plasmid-map-sequence-id-314765

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  25. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Jacob Corn
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102245-plasmid-map-sequence-id-354473

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  27. pb-CAGGS-dCas9-blasto

    Plasmid
    #218555
    Purpose
    Encodes CAG promoter-driven dCas9 with a C-terminal bipartite SV40 NLS, along with blasticidin resistance
    Depositor
    Alejandro Chavez
    Insert
    dCas9
    Use
    CRISPR; Piggybac transposition
    Tags
    2xSV40NLS
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    218555-plasmid-map-sequence-id-431930

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  28. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226429-plasmid-map-sequence-id-458099

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  30. pcU6.3_gRNA_c1

    Plasmid
    #221381
    Purpose
    Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1
    Depositor
    James Briscoe, Tatjana Sauka-Spengler
    Type
    Empty backbone
    Use
    CRISPR
    Promoter
    G. gallus U6.3
    Available Since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221381-plasmid-map-sequence-id-454411

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  31. px330_SMAD exon 2 gRNA

    Plasmid
    #68350
    Purpose
    px330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 2 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68350-plasmid-map-sequence-id-119622

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  33. px330_P53 exon 8 gRNA

    Plasmid
    #68349
    Purpose
    px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    P53 exon 8 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68349-plasmid-map-sequence-id-119621

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  34. px330_P53 exon 7 gRNA

    Plasmid
    #68351
    Purpose
    px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    P53 exon 7 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68351-plasmid-map-sequence-id-119623

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  35. TMPRSS2 g2

    Plasmid
    #153016
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153016-plasmid-map-sequence-id-298699

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  36. px330_PTEN exon 5 gRNA

    Plasmid
    #68348
    Purpose
    px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    PTEN exon 5 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68348-plasmid-map-sequence-id-119620

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  38. TMPRSS2 g3

    Plasmid
    #153017
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153017-plasmid-map-sequence-id-298697

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Showing: 7851 - 7900 of 24790 results