We narrowed to 9,353 results for: Uty

1. pcDNAintron-LASV-GPC-c3xFLAG

   Plasmid

   #215689

   Purpose

   Produces the Lassa virus glycoprotein with a c-terminal 3xFLAG tag

   Depositor

   Melinda Brindley

   Article

   Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

   Insert

   LASV GP-FLAG
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCMV-SlugCas9-P2A-puro

   Plasmid

   #214353

   Purpose

   Expresses SlugCas9 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCas9 and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-TST-EGFP-GPIDAF

   Plasmid

   #213712

   Purpose

   For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   Tags

   Twin-strep-tag and V5-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-TST-EGFP-GPIBY55

   Plasmid

   #213711

   Purpose

   For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   Tags

   Twin-strep-tag and V5-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-kcnk18

   Plasmid

   #209000

   Purpose

   Riboprobe generation

   Depositor

   GuangJun Zhang

   Article

   Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

   Insert

   kcnk18 (kcnk18 Zebrafish)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-kcnk2a

   Plasmid

   #208983

   Purpose

   Riboprobe generation

   Depositor

   GuangJun Zhang

   Article

   Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

   Insert

   kcnk2a (kcnk2a Zebrafish)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sars Cov 2 MPRO R188S mutantSARS-CoV-2 Mpro protease (R188S mutant - clinical mutant); aliases: 3CLpro, 3C-like

   Plasmid

   #204785

   Purpose

   Bacterial expression of Sars Cov 2 MPRO R188S mutant

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R188S

   Promoter

   Available since

   Jan. 23, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. ZIKV NS2B-NS3 protease (H115A- catalytically inactive); aliases: Zika Virus NS2B-NS3 fusion protein expressed with N-terminal Tw

   Plasmid

   #204791

   Purpose

   Bacterial expression of a fusion of Zika NS2B-NS3 proteins

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Zika NS2B-NS3 fusion
   

   Use

   Tags

   6 His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDY1935

   Plasmid

   #212315

   Purpose

   Express ApmFnuc protein with sumotag

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   Isvmimi
   

   Use

   Tags

   6xHis-thrombin site-TwinStrepTag-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. WNV NS2B-NS3 protease (based on PDB 2IJO, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein

    Plasmid

    #204799

    Purpose

    Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    West Nile NS2B-NS3 fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. SARS-CoV-2 Mpro protease (R188K mutant - clinical mutant); aliases: 3CLpro, 3C-like

    Plasmid

    #204770

    Purpose

    Bacterial expression of Sars Cov 2 MPRO R188K mutant

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R188K col1

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAAV.cc81-mut

    Plasmid

    #199745

    Purpose

    Expresses AAV2 Rep and AAV.cc81 Cap

    Depositor

    Aravind Asokan

    Article

    Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

    Insert

    AAV.cc81 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    mutated amino acids 586-592 in Cap

    Promoter

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.cc84-mut

    Plasmid

    #199746

    Purpose

    Expresses AAV2 Rep and AAV.cc84 Cap

    Depositor

    Aravind Asokan

    Article

    Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

    Insert

    AAV.cc84 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    mutated amino acids 586-592 in Cap

    Promoter

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015

    Plasmid

    #202024

    Purpose

    Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coli

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al bioRxiv 2024.03.30.587094

    Insert

    Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GvpA T6A-L40A

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. p15a_AcCas12n

    Plasmid

    #203806

    Purpose

    p15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcCas12n
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    rpsl

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRRL-VinculinI997A_TurboID_Venus

    Plasmid

    #200273

    Purpose

    Lentivirus vector expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter Venus

    Depositor

    Brenton Hoffman

    Article

    Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

    Insert

    VinculinI997A_TurboID_Venus
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJUMP24_T24_pRham-ilvA-relE

    Plasmid

    #201531

    Purpose

    Expressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Dan Park

    Article

    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

    Insert

    ilvA-relE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PrhaBAD (rhamnose)

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJUMP24_T24_pRham-ilvA-relE_RBS3

    Plasmid

    #201532

    Purpose

    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Dan Park

    Article

    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

    Insert

    ilvA-relE_RBS3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PrhaBAD (rhamnose)

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Dcp2 H237A-EGFP

    Plasmid

    #201425

    Purpose

    Bacterial protein expression of Dcp2 H237A

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    H237A

    Promoter

    tac

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Dhh1 25-425-EGFP

    Plasmid

    #201430

    Purpose

    Bacterial protein expression of Dhh1 25-425

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dhh1 (DHH1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 25-425 only

    Promoter

    tac

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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