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pET51b(+)_H-Luc

Plasmid

#113924

Purpose

Plasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-Luc, for BRET

Depositor

Kai Johnsson

Publication

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pKUb-Tol2

Plasmid

#85601

Purpose

Source of Tol2 transposase driven by a human ubiquitin promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Publication

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposase

Expression

Mammalian

Promoter

human Ubiquitin

Available since

Feb. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRG206

Plasmid

#64527

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Publication

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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mPapaya1-C1

Plasmid

#54722

Purpose

Localization: C1 Cloning Vector, Excitation: 530, Emission: 541

Depositor

Robert Campbell, Michael Davidson

Publication

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Type

Empty backbone

Tags

mPapaya1

Expression

Mammalian

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pXP842

Plasmid

#46068

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

ADH2

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pES001 MBP-GSK3β-HA-His

Plasmid

#154010

Purpose

Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

GSK3B (GSK3B Human)

Tags

HA tag, His6, and MBP

Expression

Bacterial

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P4:gs:nls:VPR

Plasmid

#135983

Purpose

Expresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Publication

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the VPR activation domain

Expression

Mammalian

Promoter

CMV

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-Myc-PGK-Puro-IRES-GFP

Plasmid

#75124

Purpose

Retroviral expression plasmid encoding wild type murine Myc

Depositor

Christopher Vakoc

Publication

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

myelocytomatosis oncogene (Myc Mouse)

Use

Retroviral

Tags

None

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
CMV-SpyCas9

Plasmid

#113033

Purpose

SpyCas9 expression in mammalian cells (CMV promoter driven)

Depositor

Dominik Niopek

Publication

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SpyCas9

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHaloTag-PS

Plasmid

#86626

Purpose

Expresses Akt pseudosubstrate tagged with a HaloTag

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

HaloTag-PS(RA)

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ131-STU-Lb

Plasmid

#138096

Purpose

Golden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCOM-CD63-COM

Plasmid

#138350

Purpose

Expressing COM-CD63-COM fusion protein in mammalian cells

Depositor

Baisong Lu

Publication

Yao et al J Extracell Vesicles. 2021 Mar;10(5):e12

Insert

COM-CD63-COM fusion protein

Use

CRISPR and Lentiviral

Tags

COM is fused to the N and C termini of human CD63

Expression

Mammalian

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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gag-TadCBEd

Plasmid

#225144

Purpose

Packaging plasmid for TadCBEd in v3 PE-eVLP architecture

Depositor

David Liu

Publication

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-TadCBEd

Expression

Mammalian

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_Ef1a-->flagLaM4_Notch_tTA

Plasmid

#162237

Purpose

Lentiviral vector for the expression of LaM 4 mCherry nanobody notch tTA: flagLaM4_Notch_tTA in mammalian cells

Depositor

Wendell Lim

Publication

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaM4 mCherry nanobody Notch tTA

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pKD182

Plasmid

#90952

Purpose

Expresses ThsS under the Ptac promoter

Depositor

Jeffrey Tabor

Publication

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

ThsS

Expression

Bacterial

Promoter

Ptac

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_ABE8e–superNLS

Plasmid

#232429

Purpose

Expression plasmid for the ABE8e base editor with superNLS

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

ABE8e–superNLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZ147-BvCas12b-sgRNA-scaffold

Plasmid

#122447

Purpose

Expresses sgRNA for BvCas12b in mammalian cells

Depositor

Feng Zhang

Publication

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

U6-BvCas12b-sgRNA-scaffold, CMV-mCherry

Expression

Mammalian

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKSJ340

Plasmid

#27125

Depositor

Alan Finkelstein

Publication

Jakes et al Mol Microbiol. 2010 Feb;75(3):567-78. do

Insert

Colicin Ia + Ia immunity protein

Expression

Bacterial

Available since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
ISDra2-TnpB-mCherry-U6-ωRNA*

Plasmid

#212967

Purpose

Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter

Depositor

Chunlong Xu

Publication

Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

Inserts

ISDra2-TnpB-T2A-mCherry
ωRNA*

Use

CRISPR

Tags

NLS and NLS-FLAG

Expression

Mammalian

Promoter

CAG, T7 and U6

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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MCP-eN3x9

Plasmid

#210706

Purpose

This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module

Depositor

Isaac Hilton

Publication

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

N55K in MCP

Promoter

EF1a

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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