We narrowed to 10,370 results for: plasmids 101

1. MKC106_HBB(HBA1reg-HBA1-IRES-tEPOR)

   Plasmid

   #232411

   Purpose

   AAV production plasmid for HBA+tEPOR vector from Figs. 4-6 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA+tEPOR cassette.

   Depositor

   Kyle Cromer

   Article

   Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

   Insert

   Hemoglobin Subunit Alpha 1 (HBA1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTwist-SARS-CoV-2 Spike-deltaC18 deltaE156-F157+R158G (GGA)

   Plasmid

   #212421

   Purpose

   Encodes SARS-CoV-2 spike E156-F157deletion,R158G for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike E156-F157deletion,R158G (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E156-F157deletion,R158G

   Promoter

   CAG

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1.16 F456L

   Plasmid

   #212455

   Purpose

   Encodes SARS-CoV-2 variant XBB.1.16 Spike F456L for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike XBB.1.16 F456L (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTwist-SARS-CoV-2 Spike-deltaC18 (Bluebird)

   Plasmid

   #212406

   Purpose

   Encodes SARS-CoV-2 spike T29I,T572I,D614G,Q677H,D936N for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike T29I,T572I,D614G,Q677H,D936N (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T29I,T572I,D614G,Q677H,D936N

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTwist-SARS-CoV-2 Spike-deltaC18 deltaL242-L244+R246I (ATA)

   Plasmid

   #212357

   Purpose

   Encodes SARS-CoV-2 spike deltaL242-L244, R246I for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike deltaL242-L244, R246I (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deltaL242-L244, R246I

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. R620-M18-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6 K417T/E484K/N501Y

   Plasmid

   #170202

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   K417T, E484K, N501Y

   Promoter

   CMV51

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R620-M19-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417T/E484K/N501Y

   Plasmid

   #170203

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   K417T, E484K, N501Y

   Promoter

   CMV51

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

   Plasmid

   #170191

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   K417N, E484K, N501Y

   Promoter

   CMV51

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

   Plasmid

   #131255

   Purpose

   Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmTHC_PGKneoLox2DTA.2

    Plasmid

    #112624

    Purpose

    H2BmTeal_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-mTFP1 (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    mTeal (mTFP1)

    Expression

    Mutation

    Promoter

    No Promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEHC_PGKneoLox2DTA.2

    Plasmid

    #112623

    Purpose

    H2BEmerald_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Emerald (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Emerald

    Expression

    Mutation

    Promoter

    No Promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. JD171

    Plasmid

    #229483

    Purpose

    Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, VA RNA I, L4-22k/33k
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. JD150

    Plasmid

    #229485

    Purpose

    Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, VA RNA I, E4orf6, L4-22k/33k
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KA1396_pGL4-UBCmin-luc2/Klf4-E'

    Plasmid

    #124187

    Purpose

    Luciferase reporter driven by the Klf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KA2626_pGL4-UBCmin-luc2/Klf2-D'

    Plasmid

    #124199

    Purpose

    Luciferase reporter driven by the Klf2 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf2 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. tetO7_Ef1a_OCT4

    Plasmid

    #18918

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

17. KA1401_pGL4-UBCmin-luc2/Klf4-F'

    Plasmid

    #124188

    Purpose

    Luciferase reporter driven by the Klf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KA1395_pGL4-UBCmin-luc2/Klf4-B'

    Plasmid

    #124186

    Purpose

    Luciferase reporter driven by the Klf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JD179

    Plasmid

    #229496

    Purpose

    Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes and constitutive VA RNA I gene

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, L4-22k/33k, VA RNA I
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tre (E2A, L4-22k/33k). Wt promoter (VA RNA I), ET…

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHW1385

    Plasmid

    #229879

    Purpose

    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
    

    Use

    Tags

    mScarlet-I-C1

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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