We narrowed to 22,070 results for: ESC

1. pCHMP3-NS3-Green

   Plasmid

   #223527

   Purpose

   Mammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.

   Depositor

   Winfried Weissenhorn

   Article

   Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

   Insert

   CHMP3 (CHMP3 Human)
   

   Use

   Tags

   NS3 Hepatitis C protease and mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPRKCZ-C20-EGFP (C-PRKCZ-C20)

   Plasmid

   #217761

   Purpose

   Fluorescent reporter for ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Protein kinase C zeta (PRKCZ Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C20 domain (aa 405-592)

   Promoter

   CMV

   Available since

   June 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pNES-PRKCZ-C20-EGFP (C-NES-PRKCZ-C20)

   Plasmid

   #217763

   Purpose

   Fluorescent reporter for ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Protein kinase C zeta (PRKCZ Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   NES (ALQKKLEELELDEAPVAT) plus C20 domain (aa 405-…

   Promoter

   CMV

   Available since

   June 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pEGFP-PRKCZ-C20 (N-PRKCZ-C20)

   Plasmid

   #217762

   Purpose

   Fluorescent reporter for ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Protein kinase C zeta (PRKCZ Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Linker GGSSGGGA plus C20 domain (aa 405-592)

   Promoter

   CMV

   Available since

   June 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCMV(MinDis)-iDianiSnFR_PM

   Plasmid

   #177751

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   pCMV(MinDis)-iDianiSnFR_PM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV(MinDis)-iDianiSnFR_ER

   Plasmid

   #177750

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   pCMV(MinDis)-iDianiSnFR_ER
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV(MinDis)-iCyt_BrEt_SnFR_PM

   Plasmid

   #177747

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   pCMV(MinDis)-iCyt_BrEt_SnFR_PM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. _pCMV(MinDis)-iCyt_F_SnFR_PM

   Plasmid

   #177745

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   _pCMV(MinDis)-iCyt_F_SnFR_PM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV(MinDis)-iCytSnFR_PM

   Plasmid

   #177743

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

   Depositor

   Henry Lester

   Article

   Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

   Insert

   pCMV(MinDis)-iCytSnFR_PM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV(MinDis)-iCyt_BrEt_SnFR_ER

    Plasmid

    #177746

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pCMV(MinDis)-iCyt_BrEt_SnFR_ER
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. _pCMV(MinDis)-iCyt_F_SnFR_ER

    Plasmid

    #177744

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    _pCMV(MinDis)-iCyt_F_SnFR_ER
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV(MinDis)-iCytSnFR_ER

    Plasmid

    #177742

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pCMV(MinDis)-iCytSnFR_ER
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. rat brain inositol 1,4,5-trisphosphate 3-kinase A

    Plasmid

    #122155

    Purpose

    rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)

    Depositor

    Christophe Erneux

    Article

    Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

    Insert

    inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-HA-CDK4

    Plasmid

    #172612

    Purpose

    Expresses HA-tagged CDK4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

    Plasmid

    #83842

    Purpose

    Fluorescent probe for S/G2 cell cycle transition and G2/M transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-GFP11-Clathrin light chain

    Plasmid

    #70217

    Purpose

    Expresses GFP11-clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP11-Clathrin light chain (CLTA Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSFDuet-1(nsp8-nsp7)(nsp12)

    Plasmid

    #165451

    Purpose

    Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

    Depositor

    Marc Delarue

    Article

    Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

    Inserts

    nsp12
    nsp8
    nsp7
    

    Use

    Tags

    14Histidine-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-Beatrix

    Plasmid

    #167528

    Purpose

    Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

    Depositor

    Gian Michele Ratto

    Article

    Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

    Insert

    Beatrix
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Proinsulin-NanoLuc in pLX304

    Plasmid

    #62057

    Purpose

    Luminescent reporter of insulin secretion

    Depositor

    David Altshuler

    Article

    Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

    Insert

    Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Inserted NanoLuc into C-peptide of mouse Ins2 cod…

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIG-601_NY-ESO-1_TCR_GFP

    Plasmid

    #207482

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    GFP, NY-ESO-1_TCR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Potential silent point mutation in GFP sequence

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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