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pRSETb-tVYN-TmΔ

Plasmid

#149694

Purpose

High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-TmYcgRΔ-NLucΔ4

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCZ886

Plasmid

#73584

Purpose

Pmex-5-HIS-72-miniSOG-3'UTR(tbb-2) for optogenetic mutagenesis

Depositor

Yishi Jin

Article

Noma et al Nat Commun. 2015 Dec 3;6:8868. doi: 10.1

Insert

HIS-72 (his-72 Nematode)

Use

For mossci insertion

Tags

miniSOG

Expression

Worm

Promoter

Pmex-5

Available Since

March 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLPhygCAS9

Plasmid

#63555

Purpose

Expresses CAS9 in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

Insert

Humanized Streptococcus pyogenes Cas9 from PX330 (Addgene)

Use

Leishmania expression

Tags

flag

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pSA_1_mKate2_synCoTC

Plasmid

#199470

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mKate2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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p2207 pGEM p53

Plasmid

#10853

Depositor

Peter Howley

Article

Werness et al Science. 1990 Apr 6. 248(4951):76-9.

Insert

p53 (TP53 Human)

Expression

Bacterial

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pSA_1_mTagBFP2_synCoTC

Plasmid

#199473

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mTagBFP2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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TBRE-TK-Luc-MUT

Plasmid

#36245

Depositor

Bruce Blumberg

Article

Janesick et al Development. 2012 Mar;139(6):1213-24.

Insert

Two copies of the Brachyury T-box site

Tags

Luciferase

Expression

Mammalian

Mutation

Mutations in the Brachyury palindromic element:CA…

Promoter

TK minimal promoter

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pCJ155

Plasmid

#175054

Purpose

psbEFLJ integrative plasmid expresses CRE recombinase from the rbcL promoter uses zeocin to integrate

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Promoter

rbcLXS

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pJSK619

Plasmid

#205134

Purpose

Expression of SnxA-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-BREP

Plasmid

#172340

Purpose

AAV transfer plasmid for BREP (bioluminescent red protein)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Use

AAV

Promoter

hSyn promoter

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDWJ27

Plasmid

#207604

Purpose

GFP-BIB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BIB

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMIN-0

Plasmid

#197782

Purpose

Level 2 destination vector: minimal (non-T-DNA) backbone

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pXL-CAG-ICeuI-sHRPaNRX3beta

Plasmid

#79910

Purpose

piggyBac transposon vector, sHRPaNRX3beta

Depositor

Joshua Sanes

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPaNRX3beta

Use

Piggybac

Expression

Mammalian

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)

Plasmid

#50031

Purpose

Expression of S. pyogenes SrtA at the plasma membrane in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

PM-SrtA(strep)

Tags

C-HA

Expression

Mammalian

Promoter

pCMV

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pJV0010

Plasmid

#205132

Purpose

Expression of 1xPX-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

1xPX domain of SnxA

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTGFP-T7-Hha10comp

Plasmid

#65428

Purpose

Competitor vector for transcriptional mutagenesis assay

Depositor

Yinsheng Wang

Article

You et al Nat Protoc. 2015 Sep;10(9):1389-406. doi

Type

Empty backbone

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pET21-tVYN-Nt∆

Plasmid

#154431

Purpose

Plasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detection

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-NtYcgRΔ-NLucΔ4

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMC-1-ccdB-4

Plasmid

#197814

Purpose

Level 0 J1-J4 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-ccdB-11

Plasmid

#197815

Purpose

Level 0 J1-J11 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-TetR-cryaa-YFP

Plasmid

#73202

Purpose

BAC targeting cassette for pTARBAC2 with tetR selection marker and lens-specific transgenesis marker Citrine

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

YFP

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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