We narrowed to 6,293 results for: cat.2

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pGR-L3S2P00

Plasmid

#46005

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

L3S2P00 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGR-L3S2P36

Plasmid

#46007

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

L3S2P36 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pDD143

Plasmid

#119880

Purpose

Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pBAD_dCas9sth1

Expression

Bacterial

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMLS236

Plasmid

#73684

Purpose

SapTrap 3-site Destination vector for internal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS235

Plasmid

#73683

Purpose

SapTrap 3-site Destination vector for C-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTET2 (CD)

Plasmid

#89736

Purpose

Expresses the CD-domain truncation to mouse TET2 in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

TET2 (Tet2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

CD (cysteine-rich, dioxygenase domain) truncation…

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTET2 (N1041)

Plasmid

#89737

Purpose

Expresses the N1041 truncation to mouse TET2 in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

TET2 (Tet2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

N-term 1041 truncation of mouse TET2 (contains fi…

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTET2 (N500)

Plasmid

#89897

Purpose

Expresses mouse TET2 N500 truncation in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

TET2 (Tet2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

N-term 500 truncation of mouse TET2 (contains fir…

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-C

Plasmid

#156471

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-C (ORF1ab Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET Trx His6 LIC cloning vector (2Tc-T)

Plasmid

#37239

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis and Thioredoxin (Trx)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

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f2H ∂HD (272)

Plasmid

#24428

Depositor

Steven Rosen

Article

Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

Insert

SULF2 (SULF2 Human)

Tags

FLAG, His, and Myc

Expression

Mammalian

Mutation

AA 418-716 deleted, 23AA N-terminal truncation

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
Ssh2 gRNA#1

Plasmid

#163397

Purpose

Cas9-mediated knockout of Ssh2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh2 (Ssh2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh2 gRNA#3

Plasmid

#163399

Purpose

Cas9-mediated knockout of Ssh2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh2 (Ssh2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pPN458

Plasmid

#137876

Purpose

PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGex-4T-2_LC3A

Plasmid

#73946

Purpose

For recombinant expression of human LC3A/MAP1LC3A in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

LC3A (MAP1LC3A Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pGex-4T-2_GEC1

Plasmid

#73945

Purpose

For recombinant expression of human GEC1/GABARAPL1 in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

GEC1 (GABARAPL1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pPN459

Plasmid

#137875

Purpose

PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAc-GFP-dSUMOdeltaC

Plasmid

#85697

Purpose

expression of GFP-SUMO1 delta C in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Article

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

SUMO (Sumo Fly)

Tags

GFP

Expression

Insect

Mutation

non-conjugatable SUMO, lacking the C-terminal gly…

Promoter

pAc5

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pET GFP LIC cloning vector (2GFP-T)

Plasmid

#29716

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, SiriusGFP, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-FRNK

Plasmid

#74481

Purpose

This plasmid was constructed in order to characterize the function of Proline-rich tyrosine kinase 2 (PYK2).

Depositor

J Thomas Parsons

Article

Xiong et al J Cell Biol. 1997 Oct 20;139(2):529-39.

Insert

FRNK (PTK2 Chicken)

Tags

c-myc

Expression

Mammalian

Mutation

FRNK is the C-terminal, noncatalytic domain of th…

Promoter

CMV

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only