We narrowed to 505 results for: SPY

1. dSpyCas9-p300-P2A-CASANOVA

   Plasmid

   #113129

   Purpose

   Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   dCas9-p300-P2A-CASANOVA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFBD-SpyTag-noro-VLP

   Plasmid

   #165989

   Purpose

   Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

   Depositor

   Vesa Hytönen

   Article

   Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

   Insert

   SpyTag-noro-VLP (ORF2 Synthetic, norovirus)
   

   Use

   Tags

   SpyTag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-Talin head-SpyCatcher003

   Plasmid

   #133566

   Purpose

   Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   EGFP-Talin head-SpyCatcher003
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSFFV-SpyTag-sfCherry2(11)-TagBFP

   Plasmid

   #117485

   Purpose

   Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   SpyTag002-GG-sfCherry2(11)
   

   Use

   Lentiviral

   Tags

   TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEC3109 (pSpy1C-Plac(1)_ffluc)

   Plasmid

   #218514

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   deletion of mrfp cassette, mutagenesis of the RBS…

   Promoter

   Plac(1)

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEC3111 (pSpy1C-Plac(3)_ffluc)

   Plasmid

   #218778

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   deletion of mrfp cassette, mutagenesis of the RBS…

   Promoter

   Plac(3)

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpyTag003-Talin rod-mCherry

   Plasmid

   #133567

   Purpose

   Expresses SpyTag003-Talin rod-mCherry in mammalian cells

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-Talin rod-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a SnoopTag-mEGFP-SpyTag

   Plasmid

   #72325

   Purpose

   Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a SnoopTag-mEGFP-SpyTag
   

   Use

   Tags

   N-terminal His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21b-MBPx-MBPx-SpyTag003

   Plasmid

   #216291

   Purpose

   Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   MBPx-MBPx-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 and SpyTag003

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE80L-SpyCatcher-ELP-GFP

    Plasmid

    #69835

    Purpose

    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

    Insert

    SpyCatcher-ELP-GFP
    

    Use

    Tags

    6x His Tag, GFP, and TEV Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter/lac operator element

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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