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We narrowed to 798 results for: Numb

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Showing: 601 - 640 of 798 results

  2. pB6-Csrp2-T2A-sfGFP-iCre-ERT2-FNF-DTA

    Plasmid
    #140270
    Purpose
    B6J Csrp2-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Csrp2 (Csrp2 Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    C-terminal fusion of T2A-sfGFP-iCre-ERT2
    Expression
    Mammalian
    Available Since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    140270-plasmid-map-sequence-id-317909

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  3. RAB14 3'UTR D1D2

    Plasmid
    #61492
    Purpose
    luciferase reporter containing RAB14 3' UTR with both predicted MiR144 binding site deleted
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    RAB14 3' UTR (RAB14 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    contains position 837-1637 (numbering relative to…
    Promoter
    PGK
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pCALNL_chr13_102010574-102010650

    Plasmid
    #81213
    Purpose
    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    gix-Neo-gix deletion cassette upstream of EGFP
    Expression
    Mammalian
    Promoter
    pCBa
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81213-plasmid-map-sequence-id-163268

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  5. pCALNL_Ch12_62418577-62418652

    Plasmid
    #81212
    Purpose
    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 12 (numbers in common name refer to pos. in hg38)
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    gix-Neo-gix deletion cassette upstream of EGFP
    Expression
    Mammalian
    Promoter
    pCBa
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81212-plasmid-map-sequence-id-163264

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  6. RAB14 3'UTR D1

    Plasmid
    #61490
    Purpose
    luciferase reporter containing RAB14 3' UTR with the first predicted MiR144 binding site deleted
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    RAB14 3' UTR (RAB14 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    contains position 837-1637 (numbering relative to…
    Promoter
    PGK
    Available Since
    Jan. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. RAB14 3'UTR D2

    Plasmid
    #61491
    Purpose
    luciferase reporter containing RAB14 3' UTR with the second predicted MiR144 binding site deleted
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    RAB14 3' UTR (RAB14 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    contains position 837-1637 (numbering relative to…
    Promoter
    PGK
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pS44i8GH-2

    Plasmid
    #207526
    Purpose
    Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP
    Use
    CRISPR; Pseudomonas vector
    Available Since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207526-plasmid-map-sequence-id-409598

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  10. pB6-Id1-loxP-AU1-rox-FNF-loxP-drePBD*-rox

    Plasmid
    #168770
    Purpose
    B6J Id1 AU1 tag and LSL-TagRFP-T-drePBD* targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1 (Id1 Mouse)
    Use
    Cre/Lox and Mouse Targeting; Dre / rox
    Mutation
    See Depositor Comments Below
    Available Since
    Aug. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    168770-plasmid-map-sequence-id-337553

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  11. pKP1693

    Plasmid
    #167595
    Purpose
    Express FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Gresock et al J Bacteriol. 2017 Apr 25;199(10). pii: J
    Insert
    FepA
    Expression
    Bacterial
    Mutation
    His6 tag inserted after residue 393 in the mature…
    Available Since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167595-plasmid-map-sequence-id-332904

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  12. pB6-Cdk6-T2A-TagRFPT-dre-PBD*-FNF-TK

    Plasmid
    #141452
    Purpose
    B6J Cdk6-T2A-TagRFPT-dre-PBD* allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Cdk6 (Cdk6 Mouse)
    Use
    Mouse Targeting; Dre / rox
    Tags
    C-terminal fusion of T2A-TagRFPT-dre-PBD*
    Expression
    Mammalian
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    141452-plasmid-map-sequence-id-315092

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  14. pCALNL_Ch5_155183064-155183141

    Plasmid
    #81211
    Purpose
    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 5 (numbers in common name refer to pos. in hg38)
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    gix-Neo-gix deletion cassette upstream of EGFP
    Expression
    Mammalian
    Promoter
    pCBa
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81211-plasmid-map-sequence-id-163261

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  15. pAC-BETA

    Plasmid
    #53272
    Purpose
    Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.
    Insert
    crtE, crtY, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53272-plasmid-map-sequence-id-487543

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  18. pM9-BetaGal-8C

    Plasmid
    #172489
    Purpose
    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    M9-BetaGal-8C
    Tags
    6xHis and M9 nuclear localization sequence (from …
    Expression
    Bacterial
    Mutation
    BetaGal has two cysteine mutations (C77A, C1022S)…
    Promoter
    T7
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    172489-plasmid-map-sequence-id-356067

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  19. pS44i8GM

    Plasmid
    #207527
    Purpose
    Conditionally-replicating in Pseudomonas vector, medium-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP
    Use
    CRISPR; Pseudomonas vector
    Available Since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207527-plasmid-map-sequence-id-409603

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  33. pETDuet-solo-NHis6-NocO-D282A

    Plasmid
    #240407
    Purpose
    expresses N-His tagged NocO D282A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Asp…
    Promoter
    T7
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240407-plasmid-map-sequence-id-485005

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  34. pETDuet-solo-NHis6-NocO-H286A

    Plasmid
    #240409
    Purpose
    expresses N-His tagged NocO H286A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, His…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240409-plasmid-map-sequence-id-478125

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  35. pETDuet-solo-NHis6-NocO-H132A

    Plasmid
    #240404
    Purpose
    expresses N-His tagged NocO H132A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, His…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240404-plasmid-map-sequence-id-478123

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  36. pETDuet-solo-NHis6-NocO-E129A

    Plasmid
    #240403
    Purpose
    expresses N-His tagged NocO E129A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240403-plasmid-map-sequence-id-478122

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  37. pETDuet-solo-NHis6-NocO-Q128A

    Plasmid
    #240402
    Purpose
    expresses N-His tagged NocO Q128A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240402-plasmid-map-sequence-id-478121

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  38. pETDuet-solo-NHis6-NocO-E252A

    Plasmid
    #240405
    Purpose
    expresses N-His tagged NocO E252A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240405-plasmid-map-sequence-id-478119

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  39. pETDuet-solo-NHis6-NocO-E283A

    Plasmid
    #240408
    Purpose
    expresses N-His tagged NocO E283A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240408-plasmid-map-sequence-id-478118

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  40. pETDuet-solo-NHis6-NocO-H279A

    Plasmid
    #240406
    Purpose
    expresses N-His tagged NocO H279A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, His…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240406-plasmid-map-sequence-id-478117

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