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We narrowed to 1,374 results for: ccdB

Showing: 601 - 640 of 1374 results

  1. pMOD_B2203

    Plasmid
    #91066
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CmYLCV
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91066-plasmid-map-sequence-id-181864

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  2. pMOD_C2515

    Plasmid
    #91083
    Purpose
    Module C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    AtU6
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91083-plasmid-map-sequence-id-182047

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  3. pMOD_C2518

    Plasmid
    #91087
    Purpose
    Module C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    TaU6
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91087-plasmid-map-sequence-id-182052

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  4. pMOD_C2616

    Plasmid
    #91090
    Purpose
    Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    At7SL
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91090-plasmid-map-sequence-id-182055

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  5. pMOD_C2517

    Plasmid
    #91086
    Purpose
    Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    TaU3
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91086-plasmid-map-sequence-id-182051

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  6. pMDC32B-AtTAS1c-D2-B/c

    Plasmid
    #137884
    Purpose
    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor into position downstream 3'D1[+]
    Depositor
    Alberto Carbonell
    Article
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Insert
    AtTAS1c-D2-B/c
    Use
    Tags
    Expression
    Plant
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    2x35S
    Available Since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137884-plasmid-map-sequence-id-275811

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  7. pH7WG2B-OsMIR390-B/c

    Plasmid
    #61465
    Purpose
    Plant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.
    Depositor
    James Carrington
    Article
    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1
    Insert
    OsMIR390-B/c
    Use
    Tags
    Expression
    Plant
    Mutation
    O. sativa MIR390 precursor sequence including a c…
    Promoter
    OsUBI
    Available Since
    March 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    61465-plasmid-map-sequence-id-100075

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  8. pMOD_C2520

    Plasmid
    #91089
    Purpose
    Module C, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    OsU6
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91089-plasmid-map-sequence-id-182054

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  10. pMDC123SB-OsMIR390-B/c

    Plasmid
    #61466
    Purpose
    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.
    Depositor
    James Carrington
    Article
    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1
    Insert
    OsMIR390-B/c
    Use
    Tags
    Expression
    Plant
    Mutation
    O. sativa MIR390 precursor sequence including a c…
    Promoter
    2x35S
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61466-plasmid-map-sequence-id-100072

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  11. pMOD_C2516

    Plasmid
    #91084
    Purpose
    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    At7SL
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91084-plasmid-map-sequence-id-182048

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  12. pENTR-AtTAS1c-D2-B/c

    Plasmid
    #137883
    Purpose
    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]
    Depositor
    Alberto Carbonell
    Article
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Insert
    AtTAS1c-D2-B/c
    Use
    Gateway-compatible entry clone
    Tags
    Expression
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available Since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137883-plasmid-map-sequence-id-275808

    Enlarge

  13. pMOD_C2516b

    Plasmid
    #91085
    Purpose
    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    At7SL
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91085-plasmid-map-sequence-id-182050

    Enlarge

  14. pMOD_C2519

    Plasmid
    #91088
    Purpose
    Module C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    OsU3
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91088-plasmid-map-sequence-id-182053

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  17. pUC-L3L2

    Plasmid
    #114019
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114019-plasmid-map-sequence-id-222772

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  18. pUC-L1L4

    Plasmid
    #114018
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114018-plasmid-map-sequence-id-222765

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  19. pUC-L1L2

    Plasmid
    #114017
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114017-plasmid-map-sequence-id-222768

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  20. pANY4

    Plasmid
    #164897
    Purpose
    Two valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164897-plasmid-map-sequence-id-327986

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  21. pDEST-Spe4-Kozak-miRFP713-RfA

    Plasmid
    #186381
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    186381-plasmid-map-sequence-id-392698

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  22. pDEST-Spe4-RfA-miRFP713-stop

    Plasmid
    #186391
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186391-plasmid-map-sequence-id-373838

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  23. C79m

    Plasmid
    #120991
    Purpose
    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdA antitoxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120991-plasmid-map-sequence-id-236074

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  24. pDEST-Spe4-RfA-emiRFP703-stop

    Plasmid
    #186386
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter emiRFP703 into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186386-plasmid-map-sequence-id-373831

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  25. pDEST-Spe4-Kozak-emiRFP703-RfA

    Plasmid
    #186376
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter emiRFP703 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    186376-plasmid-map-sequence-id-398283

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  26. pDEST-Spe4-RfA-mVenus-stop

    Plasmid
    #186394
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mVenus into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    186394-plasmid-map-sequence-id-392701

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  27. pDEST-Spe4-RfA-mNeonGreen-stop

    Plasmid
    #186392
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mNeonGreen into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186392-plasmid-map-sequence-id-373841

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  28. pDEST-Spe4-RfA-mEos4b-stop

    Plasmid
    #186390
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mEos4b into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186390-plasmid-map-sequence-id-373835

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  29. pDEST-Spe4-RfA-mClover3-stop

    Plasmid
    #186389
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mClover3 into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186389-plasmid-map-sequence-id-373834

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  30. pDEST-Spe4-Rfa-mCherry-stop

    Plasmid
    #186388
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mCherry into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186388-plasmid-map-sequence-id-373832

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  31. pDEST-Spe4-Kozak-mVenus-RfA

    Plasmid
    #186384
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mVenus and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186384-plasmid-map-sequence-id-373827

    Enlarge

  32. pDEST-Spe4-Kozak-mNeonGreen-RfA

    Plasmid
    #186382
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mNeonGreen and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    186382-plasmid-map-sequence-id-373805

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  33. pDEST-Spe4-Kozak-mEos4b-RfA

    Plasmid
    #186380
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mEos4b and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    186380-plasmid-map-sequence-id-373797

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  34. pDEST-Spe4-Kozak-mClover3-RfA

    Plasmid
    #186379
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mClover3 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186379-plasmid-map-sequence-id-373710

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  35. pDEST-Spe4-Kozak-mCherry-RfA

    Plasmid
    #186378
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mCherry and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186378-plasmid-map-sequence-id-373704

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  36. pDEST-Spe4-RfA-Halotag-stop

    Plasmid
    #186387
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Halo tag into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    186387-plasmid-map-sequence-id-373586

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  37. pDEST-Spe4-Kozak-Snaptag-RfA

    Plasmid
    #186385
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter Snap tag and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186385-plasmid-map-sequence-id-373828

    Enlarge

  38. pDEST-Spe4-Kozak-Halotag-RfA

    Plasmid
    #186377
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    186377-plasmid-map-sequence-id-392694

    Enlarge

  39. pDEST-Spe4-RfA-Snaptag-stop

    Plasmid
    #186395
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Snap tag into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186395-plasmid-map-sequence-id-373843

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  40. pANY8

    Plasmid
    #164900
    Purpose
    Containing His-ELP-intein multifunctional tag for protein purification by combining Ni-NTA-based and ELP-based strategy with a specially designed purification protocol.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-ELP-Intein-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    164900-plasmid-map-sequence-id-330483

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Showing: 601 - 640 of 1374 results