We narrowed to 9,390 results for: SUB

1. pHEN2-SG4 R105A

   Plasmid

   #196072

   Purpose

   Express mutant (R105A) SG4 in E.coli

   Depositor

   Shankar Balasubramanian

   Article

   Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

   Insert

   SG4 R105A
   

   Use

   Tags

   3XFLAG and 6XHis

   Expression

   Bacterial

   Mutation

   R105A mutation in SG4

   Promoter

   T7

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. V247 pCEP 4HA B56gamma1

   Plasmid

   #14534

   Depositor

   David Virshup

   Article

   Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

   Insert

   PPP2R5C B56 gamma1 regulatory subunit of PP2A (PPP2R5C Human)
   

   Use

   Tags

   4 HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. V248 pCEP 4HA B56gamma3

   Plasmid

   #14535

   Depositor

   David Virshup

   Article

   Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

   Insert

   PPP2R5C B56 gamma3 regulatory subunit of PP2A (PPP2R5C Human)
   

   Use

   Tags

   4 HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mTagBFP2-Endosomes-14

   Plasmid

   #55293

   Purpose

   Localization: Endosomes, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   Endosomes (RHOB Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. V245 pCEP-4HA B56alpha

   Plasmid

   #14532

   Depositor

   David Virshup

   Article

   Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

   Insert

   PPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)
   

   Use

   Tags

   4 HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPAmCherry1-N1

   Plasmid

   #31928

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

   Insert

   PAmCherry1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

   Promoter

   cmv

   Available since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSWEET-bgaB

   Plasmid

   #210272

   Purpose

   Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

   Depositor

   Eric Brown

   Article

   Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

   Insert

   bgaB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PxylA

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mTagBFP2-SiT-N-15

   Plasmid

   #55325

   Purpose

   Localization: Golgi TGN, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   SiT (ST6GAL1 Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   aa 1-45 of SiT

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pcDNA3-10His-StayGold

   Plasmid

   #211362

   Purpose

   Mammalian expression of the bright and photostable StayGold fluorescent protein. Contains a N-terminal 10xHis tag.

   Depositor

   Mohan Balasubramanian

   Article

   Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

   Insert

   StayGold
   

   Use

   Tags

   10xHis-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBAD/HisD-rsTagRFP

    Plasmid

    #31927

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

    Insert

    rsTagRFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

    Promoter

    araB

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPAmCherry1-C1

    Plasmid

    #31929

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mTagBFP2-PCNA-19-SV40NLS-4

    Plasmid

    #55320

    Purpose

    Localization: Nucleus, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Use

    Tags

    3xSV40 NLS and mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. VKG1-gRNA-pX330

    Plasmid

    #108671

    Purpose

    Cleavage of VKG1 sequence by CRISPR/Cas9

    Depositor

    Shigeo Sugano

    Article

    Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

    Insert

    gRNA for VKG1 sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVKG1-Puro

    Plasmid

    #108670

    Purpose

    Puromycin resistant cassette for knock-in by VIKING method

    Depositor

    Shigeo Sugano

    Article

    Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

    Insert

    PURO
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFUdioFRTeYFPW

    Plasmid

    #133998

    Purpose

    expresses eYFP upon coexpression with the recombinase flippase (Flp)

    Depositor

    Elly Nedivi

    Article

    Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

    Insert

    eYFP
    

    Use

    Lentiviral; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    inverted and flanked by two incompatible FRT site…

    Promoter

    UbC

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. V249 pCEP-4HA B56delta

    Plasmid

    #14536

    Depositor

    David Virshup

    Article

    Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

    Insert

    PPP2R5D B56 delta regulatory subunit of PP2A (PPP2R5D Human)
    

    Use

    Tags

    4 HA

    Expression

    Mammalian

    Mutation

    R210H

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. YFP Gamma 9

    Plasmid

    #36107

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 9
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    HIs tag between YFP and insert

    Promoter

    CMV

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

18. V250 pCEP-4HA B56epsilon

    Plasmid

    #14537

    Depositor

    David Virshup

    Article

    Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

    Insert

    PPP2R5C B56 epsilon regulatory subunit of PP2A (PPP2R5E Human)
    

    Use

    Tags

    4 HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pKS235

    Plasmid

    #191855

    Purpose

    Negative selection marker for MosSCI

    Depositor

    K Subramaniam

    Article

    Revathi et al MicroPubl Biol. 2022 Oct 20;2022:10.1791

    Insert

    peel-1 toxic protein for negative selection against extrachromosomal array
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    hsp-16.41

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTagBFP2-Vinculin-23

    Plasmid

    #55334

    Purpose

    Localization: Focal Adhesions, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    V234L in Vinculin.

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

21. nonphosphorylatable Mis12-targeted Aurora B FRET sensor

    Plasmid

    #45229

    Depositor

    Michael Lampson

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    non phosphorylatable Mis12-targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T-A mutation in substrate sequence

    Promoter

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAGGS-rat PKA-RIIbeta-mEGFP

    Plasmid

    #45530

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RII beta tagged by mEGFP
    

    Use

    With chicken beta actin promoter and wpre

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBABE hygro rat IRS-3

    Plasmid

    #11450

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-3 (Irs3 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

24. BiFC3

    Plasmid

    #190048

    Purpose

    BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)

    Depositor

    Patrick Van Dijck

    Article

    Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

    Insert

    YemVenus (ATG-aa173-238)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Met3

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BiFC4

    Plasmid

    #190049

    Purpose

    BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)

    Depositor

    Patrick Van Dijck

    Article

    Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

    Insert

    YemVenus (ATG-aa173-238)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Met3

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BiFC2

    Plasmid

    #190047

    Purpose

    BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)

    Depositor

    Patrick Van Dijck

    Article

    Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

    Insert

    YemVenus (aa1-172 - TAA)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Met3

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BiFC1

    Plasmid

    #190046

    Purpose

    BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

    Depositor

    Patrick Van Dijck

    Article

    Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

    Insert

    YemVenus (aa1-172)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Met3

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJet1.2 Tev-Halo tag

    Plasmid

    #117124

    Purpose

    C-terminal Tev Halo-Tag

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    Tev-Halo-tag Tag
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Tags

    Tev-Halo-Tag

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Myc-gamma3 (in pCDNA3.1)

    Plasmid

    #118041

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Myc-gamma1 (in pCDNA3.1)

    Plasmid

    #118038

    Purpose

    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG1 (Gabrg1 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Myc-gamma3 (in pCAGGS)

    Plasmid

    #118042

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pZS*11-yfp0

    Plasmid

    #53241

    Purpose

    express yellow fluorescent protein (YFP) with strong T7 RBS at a low gene dosage (3-4 copies/cell)

    Depositor

    Philippe Cluzel

    Article

    Subramaniam et al Proc Natl Acad Sci U S A. 2013 Feb 5;110

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtetO1

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. nonphosphorylatable Cenp-B-targted Aurora B FRET sensor

    Plasmid

    #45230

    Depositor

    Michael Lampson

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    nonphos Cenp-B targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    t-a mutation in substrate sequence

    Promoter

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Myc-gamma2s-EGFP

    Plasmid

    #118040

    Purpose

    GABAA receptor expression (gamma2 short subunit) double tagged, near N terminus and at C-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG2 (Gabrg2 Rat)
    

    Use

    Tags

    EGFP and cMyc epitope EQKLISEEDL inserted between…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBABE puro rat IRS-3

    Plasmid

    #11362

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-3 (Irs3 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

36. pHPHS853

    Plasmid

    #228236

    Purpose

    Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible EYFP reporter for integration into Bxb1 attP_GA landing pad

    Depositor

    Arvind Rasi Subramaniam

    Article

    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

    Inserts

    mCherry
    EYFP
    

    Use

    Tags

    HA and PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GV and none

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGADT7-AP-4 β4

    Plasmid

    #198327

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-4 β4
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Contains L480S substitution

    Promoter

    ADH1

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGADT7-AP-1 σ1C

    Plasmid

    #198336

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-1 σ1C
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    S148C substitution

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Myc-gamma1 (in pCAGGS)

    Plasmid

    #118039

    Purpose

    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG1 (Gabrg1 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MPS-3

    Plasmid

    #113071

    Purpose

    Expresses worm MPS-3 in mammalian cells

    Depositor

    Federico Sesti

    Article

    Park et al J Biol Chem. 2005 Jun 10;280(23):21893-9

    Insert

    MiRP K+ channel accessory Subunit 3 (mps-3 Nematode)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pOX + JShal XR

    Plasmid

    #16148

    Depositor

    Larry Salkoff

    Article

    Jegla et al J Neurosci. 1997 Jan 1. 17(1):32-44.

    Insert

    Polyorchis penicillatus potassium channel gamma subunit (LOC721209, Ggamma1 jelly fish)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

42. BS.YxiN.Twin1.a1-368.wt

    Plasmid

    #13528

    Depositor

    David McKay

    Article

    Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

    Insert

    B. subtilis DEAD-box helicase YxiN first two domains
    

    Use

    Tags

    intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    deleted amino acid residues 369-479

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. BS.YxiN.Twin1.a404-479.wt

    Plasmid

    #13529

    Depositor

    David McKay

    Article

    Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

    Insert

    B. subtilis DEAD-box helicase YxiN specific RNA binding domain
    

    Use

    Tags

    intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    delete amino acid residues 1-403

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAR357_SFP_pCDF-1b

    Plasmid

    #122851

    Purpose

    Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    Sfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Red cGull

    Plasmid

    #191853

    Purpose

    Expresses biosensor for cGMP in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

    Insert

    Red cGull
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET-MCN-10His-StayGold

    Plasmid

    #211360

    Purpose

    Bacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    StayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Red Glifon 300

    Plasmid

    #163115

    Purpose

    Red fluorescent protein-based glucose sensor for mammalian cells with higer affinity

    Depositor

    Takashi Tsuboi

    Article

    Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

    Insert

    Red Glifon 300
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET-MCN-10His-mStayGold (E138D)

    Plasmid

    #211361

    Purpose

    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    E138D

    Promoter

    T7

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. Red Glifon 3000

    Plasmid

    #163116

    Purpose

    Red fluorescent protein-based glucose sensor for mammalian cells with lower affinity

    Depositor

    Takashi Tsuboi

    Article

    Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

    Insert

    Red Glifon 3000
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. qi5

    Plasmid

    #24501

    Depositor

    Bruce Conklin

    Article

    Conklin et al Nature. 1993 May 20. 363(6426):274-6.

    Insert

    Gq alpha (Gnaq Mouse)
    

    Use

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    Gq alpha with the C-terminal amino acids changed …

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only