We narrowed to 13,664 results for: 109
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Plasmid#109412PurposeRepA MutantDepositorInsertrepA
TagsRFPExpressionBacterialMutationE83K repA mutation to increase copy numberAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
A3Ai E72A-Cas9n-UGI-NLS
Plasmid#109430PurposeExpresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.DepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
UseCRISPRExpressionMammalianMutationInsertion of an L1 intron into the coding sequenc…PromoterCMVAvailable SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT89
Plasmid#109303PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tagDepositorTypeEmpty backboneTagsTamA 1-27, 6HisExpressionBacterialPromoterT7Available SinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB026e
Plasmid#109261PurposeAccessory plasmid for PACE of MjTyrRS variantsDepositorInsertPpsp_pIII(P29*, Y184*); luxAB; PproK_TyrT(Opt, CUA);
ExpressionBacterialMutationamber codon at position 29 and 184 of gIIIPromoterPpsp and PproKAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA14_G-alpha
Plasmid#109914PurposeProtein expression and purification of GNA14_G-alphaDepositorInsertGNA14_G-alpha (GNA14 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pHH0103_S100A11_S-100
Plasmid#109804PurposeProtein expression and purification of S100A11_S-100DepositorInsertS100A11_S-100 (S100A11 Human)
ExpressionBacterialAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-CDC42-DN
Plasmid#109584PurposeMultisite gateway vector for 3' tagging with dominant negative CDC42DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-promoter-mIL-6
Plasmid#109296PurposeIL-6 promoter reporter constructDepositorInsertmurine IL-6 promoter (Il6 Mouse)
ExpressionMammalianMutationCMV promoter in pEGFP-N1 replaced with murine IL-…Promotermurine IL-6 promoterAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
BCL9-BirA*
Plasmid#109147PurposeTo generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cellsDepositorAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA11_G-alpha
Plasmid#109911PurposeProtein expression and purification of GNA11_G-alphaDepositorInsertGNA11_G-alpha (GNA11 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab5c-DN
Plasmid#109737PurposeMultisite gateway vector for 3' tagging with dominant negative rab5cDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_R46W
Plasmid#109415PurposeRepA MutantDepositorInsertrepA
TagsRFPExpressionBacterialMutationR46W repA mutation to increase copy numberAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT90
Plasmid#109304PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tagDepositorTypeEmpty backboneTagsTamA 1-27, 6HisExpressionBacterialPromoterT7Available SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDM1515
Plasmid#109000Purposesafe haven knock-in plasmid for the act5 locus, C-terminal GFP taggingDepositorTypeEmpty backboneUseSafe haven knock-in plasmid for the act5 locus, c…TagsGFPAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS-CD64-2
Plasmid#109191PurposeEncodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression systemDepositorInsertFull-length engineered CD64, derived from human CD64
TagsTwin-StrepExpressionInsectMutationengineered for efficient expression, see publicat…PromoterPolyhedrinAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ165
Plasmid#109327PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterPromoter EC1.2 enhancer fused to EC1.1 promoterAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RPA2_RPA-C
Plasmid#109795PurposeProtein expression and purification of RPA2_RPA-CDepositorInsertRPA2_RPA-C (RPA2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_AKT1_Pkinase
Plasmid#109775PurposeProtein expression and purification of AKT1_PkinaseDepositorInsertAKT1_Pkinase (AKT1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBD21_037
Plasmid#109154PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720DepositorInsertsmRFP
iRFP720
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only