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Plasmid#200427PurposeMammalian expression of fluorescently-tagged marker for autophagic vesiclesDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TalO8-GLC3-CFP
Plasmid#178307PurposeCoTrIP plasmid with GLC3 promoter driving CFP's expressionDepositorInsertGlc3pr-CFP-tAdh1
UseTagsExpressionYeastMutationPromoterGLC3 and NAAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3×FLAG-SLC35A2
Plasmid#186283Purposetransient expression of N-terminal 3×FLAG tagged SLC35A2 isoform c/UGT1 in mammalian cellsDepositorInsertSLC35A2 (SLC35A2 Human)
UseTags3×FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP.N3-myc-SLC35A2
Plasmid#186285Purposetransient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cellsDepositorInsertSLC35A2 (SLC35A2 Human)
UseTagsMYCExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStA0::SPLc3-RBSc110
Plasmid#171893PurposePromoter SPLc3 and RBSc110 in pStA0DepositorInsertPromoter SPLc3 and RBSc110
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStA0::SPLc3-RBSc249
Plasmid#171903PurposePromoter SPLc3 and RBSc249 in pStA0DepositorInsertPromoter SPLc3 and RBSc249
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStA0::SPLc3-RBSc21
Plasmid#171884PurposePromoter SPLc3 and RBSc21 in pStA0DepositorInsertPromoter SPLc3 and RBSc21
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
SLC35B2-p2A-E2Crimson
Plasmid#83941PurposeLentiviral vector expressing SLC35B2-p2A-E2CrimsonDepositorInsertSLC35B2 (SLC35B2 Human)
UseLentiviralTagsp2A-E2CrimsonExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B2 no-stop
Plasmid#123207PurposeGateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRX-No-HaloTag7-mGFP-LC3
Plasmid#184901PurposeStable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic fluxDepositorInsertLC3B
UseTagsHaloTag7 and monomeric EGFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-GFP-LC3-RFP
Plasmid#84573PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUW mCherry-GFP-LC3
Plasmid#110060PurposeTo visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)DepositorInsertmCherry-GFP-LC3
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV GFP-LC3B WT
Plasmid#123114PurposeExpresses EGFP-LC3B wild-type in mammalian cells. Fluorescent reporter for autophagosomes.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs GFP-LC3-RFP
Plasmid#117413PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.DepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-LC3 (Atg8)
Plasmid#24919DepositorInsertLC3 (MAP1LC3B Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3B G120
Plasmid#123094PurposeExpresses 3xFLAG-LC3B G120 in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTags3xFLAGExpressionMammalianMutationDeleted amino acids 121-125. Stop codon after G12…PromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3B G120A
Plasmid#123093PurposeExpresses 3xFLAG-LC3B G120A in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTags3xFLAGExpressionMammalianMutationGlycine 120 to AlaninePromoterCMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
[pSK104] pET28 SLC38A9N-His
Plasmid#136142Purposebacterial expression of SLC38A9 N term fragment (1-119)DepositorInsertSLC38A9 N term fragment (1-119) (SLC38A9 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1 (delta110)
Plasmid#71861Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, missing first 110 amino acidsPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-MAP1LC3B
Plasmid#175753PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3BDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only