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Showing: 301 - 320 of 9197 results

  9. GFP-X3

    Plasmid
    #115568
    Purpose
    Synthetic acetylation substrate consisting of GFP fusion with 3 repeats of the Gcn5 consensus motif
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Three repeats of the Gcn5 consensus motif are tag…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    115568-plasmid-map-sequence-id-226342

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  12. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid
    #114143
    Purpose
    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    114143-plasmid-map-sequence-id-224089

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  13. Ratiometric FPX sensor for ERK kinase activity

    Plasmid
    #60974
    Purpose
    For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A-WW domain-ERK substrate-ddFP B
    Use
    Tags
    ERK substrate is before ddFP copy B, WW domain is…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pETDuet-msGC-NT21

    Plasmid
    #78102
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 2…
    Promoter
    T7
    Available since
    June 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    78102-plasmid-map-sequence-id-151428

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  15. pETDuet-msGC-NT13

    Plasmid
    #75087
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 4…
    Promoter
    Available since
    May 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    75087-plasmid-map-sequence-id-148282

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  16. pCDNA3_shRNA resistant nmPKA-Cα-mEGFP

    Plasmid
    #114144
    Purpose
    The G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    mouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G2A mutation and silent mutation to resist shRNA …
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114144-plasmid-map-sequence-id-224274

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  17. pJK539

    Plasmid
    #72515
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72515-plasmid-map-sequence-id-132543

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  18. pJK549

    Plasmid
    #72520
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72520-plasmid-map-sequence-id-132604

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  19. pJK555

    Plasmid
    #72521
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72521-plasmid-map-sequence-id-132606

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  20. pJK577

    Plasmid
    #72528
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, small subunit
    Use
    Gene synthesis
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72528-plasmid-map-sequence-id-132616

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Showing: 301 - 320 of 9197 results