We narrowed to 5,121 results for: dre

1. pSAVED-CHAT-sm_PCaspase

   Plasmid

   #214048

   Purpose

   Bacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-PCaspase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R456E, D459K, D476K, R479E

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEcNucC

   Plasmid

   #214050

   Purpose

   Bacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas system

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   EcNucC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJS-BCD-Strep-SAVED-CHAT

   Plasmid

   #214030

   Purpose

   Bacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJS-BCD-Strep-dSAVED-CHAT

   Plasmid

   #214031

   Purpose

   Bacterial expression plasmid for strep-tagged catalytically dead SAVED-CHAT mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   H375A, C422A

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJS-BCD-Strep-SAVED-CHAT-sm

   Plasmid

   #214033

   Purpose

   Bacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   R456E, D459K, D476K, R479E

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJS-BCD-Strep-PCaspase

   Plasmid

   #214034

   Purpose

   Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PCaspase
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Dyn1(K44A)-mRFP

   Plasmid

   #55795

   Purpose

   Dominant negative Dynamin 1 mutant tagged with mRFP

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

   Insert

   Dynamin-1(K44A)-mRFP (Dnm1 Rat, Discosoma sp.)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Dynamin-1(K44A)-mRFP was produced from Dynamin-1(…

   Promoter

   CMV

   Available since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJS-BCD-Strep-SAVED-CHAT-cbm

   Plasmid

   #214032

   Purpose

   Bacterial expression plasmid for strep-tagged SAVED-CHAT cA3-binding pocket mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   K121E, Q122A, N274A, R276E, Y285A, F286A

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJS-BCD-Strep-dPCaspase

   Plasmid

   #214035

   Purpose

   Bacterial expression plasmid for strep-tagged catalytically dead PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PCaspase
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   H79A, C146A

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJS-BCD-Strep-PCaspase-R153A

    Plasmid

    #214036

    Purpose

    Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    PCaspase
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    R153A

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CaMKIIa-HA-KORD-IRES-mCitrine

    Plasmid

    #65418

    Purpose

    AAV packaging vector for CamKIIa driven KOR DREADD expression

    Depositor

    Bryan Roth

    Article

    Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

    Insert

    CaMKIIa-HA-KORD-IRES-mCitrine (OPRK1 Human)
    

    Use

    AAV

    Tags

    HA and mCitrine

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-dF-HA-KORD-IRES-mCitrine

    Plasmid

    #65417

    Purpose

    AAV packaging vector for Cre-dependent, hSyn driven KOR DREADD expression.

    Depositor

    Bryan Roth

    Article

    Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

    Has Service

    AAV8

    Insert

    hSyn-dF-HA-KORD-IRES-mCitrine (OPRK1 Human)
    

    Use

    AAV

    Tags

    HA and mCitrine

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. XE129 beta2AR Fz1-pcDNA3.1

    Plasmid

    #16795

    Depositor

    Randall Moon

    Article

    Liu et al Proc Natl Acad Sci U S A. 1999 Dec 7;96(

    Insert

    beta 2 AR Fz1 (Fzd1, Adrb2 Rat)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Chimera of beta 2 adrenergic receptor (extracellu…

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

14. G protein alpha-s-CFP

    Plasmid

    #55793

    Purpose

    G protein alpha-s internally tagged with ECFP and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-ECFP (Gnas Rat)
    

    Use

    Tags

    ECFP flanked by SGGGS on each side was inserted i…

    Expression

    Mammalian

    Mutation

    His was substituted for Asn164 in ECFP (Clontech)…

    Promoter

    CMV

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. G protein alpha-s-GFP

    Plasmid

    #66083

    Purpose

    G protein alpha-s internally tagged with EGFP and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-EGFP (Gnas Rat)
    

    Use

    Tags

    EGFP flanked by SGGGS on each side was inserted i…

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. G protein alpha-s-mCherry

    Plasmid

    #66968

    Purpose

    G protein alpha-s internally tagged with mCherry and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-mCherry (Gnas Rat, Discosoma sp.)
    

    Use

    Tags

    internal EE epitope (residues 189-194 in alpha- s…

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. G protein alpha-s-mCerulean

    Plasmid

    #66084

    Purpose

    G protein alpha-s internally tagged with mCerulean and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-mCerulean (Gnas Rat, Aequorea victoria)
    

    Use

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Ef1a-dDIO hChR2(H134R)-EYFP

    Plasmid

    #55640

    Purpose

    Dre-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. PRS_8-jGCaMP7b

    Plasmid

    #192594

    Purpose

    AAV-vector for fluorescent calcium measurements from noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Maheu et al bioRxiv 2025.03.26.644382

    Insert

    jGCaMP7b
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRSX8

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid

    #50468

    Purpose

    Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Insert

    rM3D-IRES-mCitrine
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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