We narrowed to 2,412 results for: organization Addgene

1. pHR-U6-sgRNA-CMV-puro

   Plasmid

   #169450

   Purpose

   backbone for sgRNA expression

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWM_12x601_193 NRL + TetO

   Plasmid

   #157812

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_tetO
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2

   Plasmid

   #164510

   Purpose

   Green glucose sensor with mRuby2 fusion, cytosolic

   Depositor

   Loren Looger

   Article

   Keller et al bioRxiv (2019) 571422; doi: https://doi.

   Has Service

   AAV9

   Insert

   (Cyto)iGlucoSnFR-mRuby2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Synthetic Construct

   Promoter

   Synapsin

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Venus-CAAX

   Plasmid

   #87612

   Purpose

   Plasma membrane-targeted Venus (YFP).

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   Venus-CAAX
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCast

   Plasmid

   #200169

   Purpose

   E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

   Depositor

   Yong-Su Jin

   Article

   Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. ePB-PURO-TT-PA-CRE

   Plasmid

   #179584

   Purpose

   ePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)

   Depositor

   Ali Hemmati Brivanlou

   Article

   De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

   Insert

   PA-MAG-CRE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTCAV-FLEx(loxP)-FlpO

   Plasmid

   #67829

   Purpose

   Cre-dependent expression of FlpO from CAV2 virus

   Depositor

   Liqun Luo

   Article

   Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

   Insert

   FlpO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hox D4a

   Plasmid

   #36469

   Depositor

   Robert Ho

   Article

   Prince et al Development. 1998 Feb . 125(3):407-20.

   Insert

   Hox D4a (hoxd4a Zebrafish)
   

   Use

   Riboprobe synthesis

   Tags

   Expression

   Bacterial

   Mutation

   Q100H, A101T, G125C and R189W

   Promoter

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pJB042 (FtsZ-mEos2)

   Plasmid

   #49764

   Purpose

   Inducible expression of FtsZ-mEos2 in bacteria

   Depositor

   Jie Xiao

   Article

   Buss et al Mol Microbiol. 2013 Sep;89(6):1099-120.

   Insert

   FtsZ-mEos2
   

   Use

   Tags

   mEos2

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221_SLC22A1

    Plasmid

    #132034

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A1 (SLC22A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pUBC_hsPUM1-iRFP670

    Plasmid

    #107609

    Purpose

    Transient expression of Pumilio1, fused to iRFP670 in mammalian cells, under a hUBC promoter

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    hsPUM1-iRFP670
    

    Use

    Synthetic Biology

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    human UBC

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 mSynCAM 4

    Plasmid

    #115499

    Purpose

    expression of mouse full-length SynCAM 4

    Depositor

    Thomas Biederer

    Article

    Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

    Insert

    Cadm4 (Cadm4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 3xHA-BioID_pAS28

    Plasmid

    #118223

    Purpose

    expresses 3xHA-tagged BioID in C. elegans intestine

    Depositor

    Jessica Feldman

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    BioID (BirA mutant)
    

    Use

    Tags

    3x HA tag

    Expression

    Worm

    Mutation

    R118G

    Promoter

    ges-1p

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-H2B-Venus

    Plasmid

    #87614

    Purpose

    Venus (YFP) fused to histone H2B.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    H2B-Venus (H2BC8 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBFv-UAS3-GFP1-10

    Plasmid

    #138398

    Purpose

    Expresses split GFP fragment (GFP1-10)

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV6M-Pak1 H83L H86L

    Plasmid

    #12211

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PBD (p21-binding domain) mutant: H83L, H86L (see …

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMTTH+eGFP-bromo5

    Plasmid

    #157804

    Purpose

    Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    eGFP-bromo5
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMTTH+eGFP-bromo1

    Plasmid

    #157805

    Purpose

    Expression of synthetic protein composed of eGFP and BRD4 bromodomain #1

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    eGFP-bromo1
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-Bgh-GFP-Farpl

    Plasmid

    #115501

    Purpose

    expression of rat Farp1

    Depositor

    Thomas Biederer

    Article

    Cheadle et al J Cell Biol. 2012 Dec 10;199(6):985-1001

    Insert

    Farp1 (Farp1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin

    Available since

    Dec. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWZL blast HA goosecoid

    Plasmid

    #14096

    Depositor

    Bob Weinberg

    Article

    Hartwell et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

    Insert

    goosecoid (Gsc Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only