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pLN352 (FuGW-S(Elk1)p-mKate2)

Plasmid

#105175

Purpose

synthetic promoter Elk1 drives mKate2 experssion

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(Elk1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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p-dCas9-p300-S1396R-S1397R-Hygro

Plasmid

#104419

Purpose

transient expression of dCas9-dp300 fusion protein

Depositor

Stephan Beck

Insert

dp300

Use

CRISPR

Expression

Mammalian

Mutation

S1396R-S1397R

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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P-del (pTransluc-miR-145-2)

Plasmid

#21500

Depositor

Kenneth Kosik

Article

Xu et al Cell. 2009 May 15. 137(4):647-58.

Insert

0.9 kb 5' of miR-145 (MIR145 Human)

Use

Luciferase

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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pLN381 (FuGW-S(NFY)p-mKate2)

Plasmid

#105179

Purpose

synthetic promoter NFY drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(NFY)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNAI Tat C30G HA (P#38)

Plasmid

#14656

Depositor

Matija Peterlin

Article

Cujec et al Mol Cell Biol. 1997 Apr . 17(4):1817-23.

Insert

Tat (tat HIV)

Tags

HA

Expression

Mammalian

Mutation

C30G C34G

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
p-dCas9-JMJD2A-S288G-T289G-Hygro

Plasmid

#104418

Purpose

transient expression of dCas9-dJMJD2A fusion protein

Depositor

Stephan Beck

Insert

dJMJD2A

Use

CRISPR

Expression

Mammalian

Mutation

S288G-T289G

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLN593 (FuGW-S(MAFK)p-mKate2)

Plasmid

#105205

Purpose

synthetic promoter MAFK drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(MAFK)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5G6G,P-J23106-geneIII

Plasmid

#80861

Purpose

Phagemid (encodes evolved cI5G6G,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6G,P

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLN397 (FuGW-S(E2F1)p-Sponge7)

Plasmid

#105181

Purpose

Module 2 - synthetic promoter E2F1 drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(E2F1)p-Sponge7

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSV CDK9 (D167N) HA (P#601)

Plasmid

#14639

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 D167N (CDK9 Human)

Tags

HA

Expression

Mammalian

Mutation

D167N. Kinase negative mutant.

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIII

Plasmid

#80864

Purpose

Phagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI4A5C6G7G,P

Expression

Bacterial

Mutation

238G to 238D (please see depositor comment below)

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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p{3P3-dsRed::FRT-RC,attB}

Plasmid

#182177

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-eGFP::FRT-RC,attB}

Plasmid

#182175

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{3P3-mTurquoise::FRT-RC,attB}

Plasmid

#182181

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

mTurquoise

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5G6T,P-J23106-geneIII

Plasmid

#80862

Purpose

Phagemid (encodes evolved cI5G6T,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6T,P

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLINK CDK9 myc (D167N) (P#1084)

Plasmid

#14637

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 D167N (CDK9 Human)

Tags

myc

Expression

Mammalian

Mutation

D167N. Kinase negative mutant.

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pEF myc Gal CDK9 (P#1475)

Plasmid

#14631

Depositor

Matija Peterlin

Article

Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

Insert

cdk9 (CDK9 Human)

Tags

Gal and myc

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
p-super-retro puro RhoGDI2 shRNA

Plasmid

#58897

Purpose

produces an shRNA against RhoGDI2

Depositor

Channing Der

Article

Stevens et al Small GTPases. 2011 Jul;2(4):202-210. Ep

Insert

RhoGDI2 (ARHGDIB Human)

Use

RNAi

Expression

Mammalian

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
pLN553 (FuGW-S(MAFK)p-Sponge7)

Plasmid

#105203

Purpose

Module 2 - synthetic promoter MAFK drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(MAFK)p-Sponge7

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pEF myc Gal cycT2b (P#1719)

Plasmid

#14600

Depositor

Matija Peterlin

Article

Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

Insert

cycT2 (CCNT2 Human)

Tags

Gal and myc

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only