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pRS424-TUB2 - human TUBB6 Cterminal tail

Plasmid

#60403

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
LSM044-2 CMV-TO-CCR7-V2-ADAR2dd(E488Q, T501A) (FLP-IN)

Plasmid

#233396

Purpose

CCR7-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR7-ADAR2dd

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available since

June 13, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0252 (PB)EF1a-FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP

Plasmid

#233443

Purpose

PiggyBac donor plasmid to overexpress FKBP-ADAR2dd (cLIDAR)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

June 13, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP

Plasmid

#233446

Purpose

PiggyBac donor plasmid to overexpress ADAR2dd-PCP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2dd-PCP-T2A-PuroR-P2A-BFP

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

Plasmid

#233442

Purpose

PiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0250 (PB)EF1a-CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP

Plasmid

#233441

Purpose

PiggyBac donor plasmid to overexpress CCR6-ADAR2dd (gLIDAR)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0253 (PB)EF1a-ADAR2dd-MCP-T2A-PuroR-P2A-BFP

Plasmid

#233444

Purpose

PiggyBac donor plasmid to overexpress ADAR2dd-MCP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2dd-MCP-T2A-PuroR-P2A-BFP

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM043_2 (PB)SFFV-LIDARSensor(mCherry->Casp9-2A-GFP)-hygro

Plasmid

#233413

Purpose

LIDAR reporter RNA; mCherry as marker, iCasp-P2A-EGFP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

LIDARSensor(mCherry->Casp9-2A-GFP)-hygro

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ054 (PB)SFFV-LIDARsensor (hygR)

Plasmid

#233419

Purpose

PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, EGFP as output) for stable integration

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ093 SFFV-sensor-IRESv0-LIDARst(IRESv0-AVPR2)

Plasmid

#233430

Purpose

LIDAR all components on single transcript (as shown in Figure 4A), using EMCV IRES to separate each component

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

sensor-IRESv0-LIDARst(IRESv0-AVPR2)

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ098 SFFV-mCherry-LIDARsensor(W)-hIgG1-TM

Plasmid

#233429

Purpose

Positive control LIDAR reporter RNA (UAG stop codon replaced with UGG) with a membrane-bound human IgG as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-LIDARsensor(W)-hIgG1-TM

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM041-3 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-EGFP

Plasmid

#233408

Purpose

LIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, EGFP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xPP7))cSG-EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ392 SFFV-secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP

Plasmid

#233415

Purpose

LIDAR reporter RNA; secreted NanoLuc Luciferase as marker, SEAP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ400 SFFV-mCherry-SGc(stem-1(3xMS2))cSG-TEVP (equivalent to EK0461)

Plasmid

#233414

Purpose

LIDAR reporter RNA; mCherry as marker, TEVP as output (equivalent to EK0461)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2))cSG-TEVP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM053 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-mtagBFP (FLP-IN)

Plasmid

#233409

Purpose

LIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, mTagBFP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xPP7))cSG-mtagBFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM075 SFFV-mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP (FLP-IN)

Plasmid

#233439

Purpose

SFFV driven LIDAR reporter with 2 tandem substrate stem loops

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0038 Ac5-mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Plasmid

#233410

Purpose

AC5 promoter driven LIDAR reporter RNA for drosophila cell expression (mCherry as marker, EGFP as output)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Ac5

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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YH039 PGK-TO-FKBP-tm-GS-XTEN-ADAR2(DD-E488Q-T501A)

Plasmid

#233378

Purpose

PGK promoter driven FKBP fused with ADAR2dd (E488Q,T501A), with a CD28 transmembrane domain in between

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-ADAR2dd

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

PGK

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ030 CMV-TO-AVPR2-ADAR2(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233380

Purpose

AVPR2-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and extra V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR2-ADAR2dd

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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CMVTO-hIL2

Plasmid

#233387

Purpose

secreted human IL-2

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IL-2 (IL2 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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