We narrowed to 3,452 results for: ttl

1. pPB9v1

   Plasmid

   #210028

   Purpose

   Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB12A

   Plasmid

   #210035

   Purpose

   Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas12a
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. CYFIP2.mCherry

   Plasmid

   #122052

   Purpose

   Expresses human CYFIP2 in mammalian cells.

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP2 (CYFIP2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLNCX1 HA Akt1

   Plasmid

   #15990

   Depositor

   Morris Birnbaum

   Article

   Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pEA02

   Plasmid

   #172193

   Purpose

   Suicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.

   Depositor

   Jean-Luc Pernodet

   Article

   Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

   Insert

   attL
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJRTCLCrVA010

   Plasmid

   #22843

   Depositor

   Niki Robertson

   Article

   Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

   Insert

   A DNA containing GFP
   

   Use

   Cotton expression vector

   Expression

   Bacterial

   Mutation

   Cotton Leaf Crumple Virus A component with Coat P…

   Available Since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. myc-Miro1 (lacking TM)

   Plasmid

   #127613

   Purpose

   Human Miro1 with the transmembrane deleted

   Depositor

   Josef Kittler

   Article

   Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

   Insert

   Miro1 (RHOT1 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Deletion of transmembrane domain

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJRTCLCrVA009

   Plasmid

   #22842

   Depositor

   Niki Robertson

   Article

   Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

   Insert

   A DNA containing CHLI fragment
   

   Use

   Cotton vigs vector

   Expression

   Bacterial

   Mutation

   Cotton Leaf Crumple Virus Coat Protein (AR1 gene)…

   Available Since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB9

   Plasmid

   #210036

   Purpose

   Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   puro-T2A-eGFP
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-Miro1 (184-618)

    Plasmid

    #127626

    Purpose

    Human Miro1 lacking the first GTPase domain

    Depositor

    Josef Kittler

    Article

    Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

    Insert

    Miro1 (RHOT1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    184-618 only

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-dCas13-M3nls

    Plasmid

    #155366

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYL192 (TRV1)

    Plasmid

    #148968

    Purpose

    Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

    Insert

    TRV RNA1
    

    Use

    Plant vector with trv rna1; this vector used with…

    Mutation

    P697S mutation in putative replicase. S239G and S…

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CYFIP1.mCherry

    Plasmid

    #122051

    Purpose

    Expresses human CYFIP1 in mammalian cells.

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. SPDK3876(TRV-RNA2-pPEBV-MCS)

    Plasmid

    #149275

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter
    

    Use

    T-dna vector

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST47-CYFIP1.GFP

    Plasmid

    #109139

    Purpose

    GFP-tagged CYFIP1 expression construct

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB1163

    Plasmid

    #210032

    Purpose

    Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas11-P2A-Cas6-T2A-Cas3
    rtta-T2A-blast
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDNR-SpCas9-Cdt1

    Plasmid

    #80426

    Purpose

    Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Cdt1
    

    Use

    CRISPR

    Tags

    Cdt1

    Mutation

    codon optimized for human

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGMβ1

    Plasmid

    #216202

    Purpose

    Chromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.

    Depositor

    Yasuko Sasaki

    Article

    Iwamoto et al Biosci Microbiota Food Health. 2022;41(1

    Type

    Empty backbone

    Use

    Shuttle vector, conjugal plasmid, theta type rep…

    Expression

    Bacterial

    Promoter

    lac promoter in pGEM-Teasy,　Promoters in pAMbeta1…

    Available Since

    Jan. 21, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. myc-KIF5A

    Plasmid

    #127616

    Purpose

    N-terminal myc-tagged mouse KIF5A

    Depositor

    Josef Kittler

    Article

    Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

    Insert

    KIF5A (Kif5a Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST47-CYFIP2.GFP

    Plasmid

    #109140

    Purpose

    GFP-tagged CYFIP2 expression construct

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP2 (CYFIP2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge