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pKAR2-Br512g3.2

Plasmid

#171201

Purpose

Expresses Br512g3.2 polymerase in E.coli

Depositor

Andrew Ellington

Article

Bhadra et al medRxiv 2022.08.09.22278605

Insert

Engineered Bst DNA Polymerase I Large Fragment

Tags

8x His tag; Villin Headpiece 47 amino acid (vHP47…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pROSA26-5'

Plasmid

#21715

Depositor

Philippe Soriano

Article

Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

Insert

ROSA26-5' insert (Gt(ROSA)26Sor Mouse)

Use

For use with mouse targeting vector prosa26-1

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pKTol2C-EGFP

Plasmid

#85598

Purpose

Tol2 transposon with EGFP driven by minimal-CAGs promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposon with EGFP

Expression

Mammalian

Promoter

mini-CAG

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pROSA26-SAT

Plasmid

#208339

Purpose

ROSA26 targeting vector for the SAT sensor for both ERK1/2 and AKT signaling

Depositor

Philippe Soriano

Article

Dinsmore et al Dev Biol. 2023 Sep 1:S0012-1606(23)00146

Insert

SAT sensor

Use

Mouse Targeting

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCR3.1-Luc

Plasmid

#106457

Purpose

luciferase reporter, CMV promoter

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

luciferase reporter, CMV promoter

Use

Luciferase

Expression

Mammalian

Mutation

WT promoter

Available Since

Nov. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-miR29b

Plasmid

#21121

Depositor

Joshua Mendell

Article

Hwang et al Science. 2007 Jan 5. 315(5808):97-100.

Insert

miR-29b-1 (MIR29B1 Human)

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
pHA-N1

Plasmid

#86049

Purpose

A mammalian expression vector with a HA-tag at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 H2B-mIFP T2A Mpro (C145A)

Plasmid

#163080

Purpose

Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B

Depositor

Xiaokun Shu

Insert

Inactive Mpro mutant C145A and H2B-mIFP

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG:ECFP

Plasmid

#32597

Depositor

Anna-Katerina Hadjantonakis

Article

Hadjantonakis et al BMC Biotechnol. 2002 Jun 11;2:11.

Insert

ECFP

Expression

Mammalian

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-QF

Plasmid

#24339

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QF

Tags

His and Myc

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-30uDelta13V

Plasmid

#18015

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

mglB-D154A

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Hbxadr-Hatag

Plasmid

#24930

Depositor

Xin Wang

Article

Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

Insert

HBx

Tags

HA

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pQUAST-frt-stop-frt-p65AD::CaM

Plasmid

#64716

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Inserts

p65AD::CaM
frt-stop-frt

Expression

Insect

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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P1-M-P2

Plasmid

#87091

Purpose

This is the P1-M-P2 portion of the Bcl-2 promoter with a luciferase reporter in pGL3Basic plasmid

Depositor

Yohannes Tesfaigzi

Article

Bredow et al Gene. 2007 Dec 1;404(1-2):110-6. Epub 20

Type

Empty backbone

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
pHZ081

Plasmid

#27506

Depositor

Andrew Fire

Article

Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

Insert

pcol-10::lin-4::let-858_3'UTR (lin-4 Nematode)

Expression

Worm

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pBa.NgCam.GFP

Plasmid

#45061

Depositor

Gary Banker, Marvin Bentley

Article

Burack et al Neuron. 2000 May;26(2):465-72.

Insert

NgCam (L1CAM Chicken)

Tags

eGFP

Expression

Mammalian

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pPAS-E

Plasmid

#39867

Depositor

Vladislav Verkhusha

Article

Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

Insert

iRFP protein PAS domain

Tags

E coil

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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GP734

Plasmid

#164527

Purpose

Expresses Flag-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin

Use

Lentiviral

Tags

FLAG and Ub

Mutation

WT

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGAT1

Plasmid

#22804

Depositor

Henry Lester

Article

Mager et al J Neurosci. 1996 Sep 1. 16(17):5405-14.

Insert

pcDNA3.1-mGAT1

Expression

Mammalian

Mutation

wt

Available Since

Jan. 14, 2010

Availability

Academic Institutions and Nonprofits only

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pCS2-bNWASP

Plasmid

#33019

Depositor

Marc Kirschner

Article

Lee et al Science. 2010 Sep 10;329(5997):1341-5.

Insert

N-WASP (WASL Bovine)

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only