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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,963 results for: RAP

Showing: 4771 - 4800 of 6963 results

  1. CoV2-N-G204R

    Plasmid
    #177953
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (G204R) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G204R
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177953-plasmid-map-sequence-id-348180

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  2. pBMN ltDHFR(DD)-YFP

    Plasmid
    #47076
    Purpose
    ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans
    Depositor
    Thomas Wandless
    Article
    Cho et al PLoS One. 2013 Aug 22;8(8):e72393. doi:
    Insert
    ltDHFR(DD)
    Use
    Retroviral
    Tags
    HA and YFP
    Expression
    Mutation
    amino acids mutations: R12H, N23S, G67S, V78A, E1…
    Promoter
    LTR
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47076-plasmid-map-sequence-id-67102

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  4. pMuLE ENTR 7SK-miR-30 L1-L4

    Plasmid
    #62180
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    RNAi; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pBMN YFP-ltDHFR(DD)

    Plasmid
    #47080
    Purpose
    ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans
    Depositor
    Thomas Wandless
    Article
    Cho et al PLoS One. 2013 Aug 22;8(8):e72393. doi:
    Insert
    YFP-ltDHFR(DD)
    Use
    Retroviral
    Tags
    HA and YFP
    Expression
    Mutation
    amino acids mutations: R12H, N37D, N59D, G67S, D1…
    Promoter
    LTR
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47080-plasmid-map-sequence-id-67106

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  10. DHFR-GFP-th-NL1

    Plasmid
    #173007
    Purpose
    For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    DHFR-GFP-NL1
    Use
    Tags
    DHFR and EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    173007-plasmid-map-sequence-id-339111

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  14. VanGlow-RR

    Plasmid
    #83343
    Purpose
    for Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Cheng-Yu Lee
    Article
    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    mCherry::Renilla(nls)
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83343-plasmid-map-sequence-id-158672

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  15. DHFR-GFP-th-GluA1

    Plasmid
    #173008
    Purpose
    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    DHFR-GFP-th-GluA1
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    173008-plasmid-map-sequence-id-339527

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  23. pOPO685

    Plasmid
    #195300
    Purpose
    pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade and native array with PaqCI entry site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose inducible arapBAD
    Available since
    May 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195300-plasmid-map-sequence-id-394739

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  25. AA427

    Plasmid
    #215961
    Purpose
    Fragmid fragment: (guide cassette) enables iBar UMIs
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215961-plasmid-map-sequence-id-431415

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  26. CoV2-N-D3L

    Plasmid
    #177945
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (D3L) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D3L
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177945-plasmid-map-sequence-id-348204

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  27. AA406

    Plasmid
    #216070
    Purpose
    Fragmid fragment: (2A Selection) Violet-excited GFP, T2A, blasticidin resistance
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    P2A_v1.1; VexGFP_v1.1; T2A_v1.1; BlastR_v1.1; Stop
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216070-plasmid-map-sequence-id-431465

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  29. CoV2-N-A119S

    Plasmid
    #177948
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (A119S). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (A119S) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    A119S
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177948-plasmid-map-sequence-id-348195

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Showing: 4771 - 4800 of 6963 results