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psiCHECK-EGFP

Plasmid

#196606

Purpose

Dual-Luciferase Reporter vector carrying an EGFP direct sequence

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

GFP

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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ptRNAfMet-A37G

Plasmid

#196658

Purpose

Plasmid for overexpression of mutated initiator tRNA (A37G)

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

tRNAfMet-A37G expression cassette

Expression

Bacterial

Mutation

A37G in tRNAfMet

Promoter

metY native promoter

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-MBP_H

Plasmid

#146486

Purpose

Insect Expression of MBP

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

MBP

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-V5His6_E

Plasmid

#146223

Purpose

Insect Expression of GST

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

GST

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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p09008_AF1-4+GFP5-7

Plasmid

#173715

Purpose

Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

Depositor

Johannes Kabisch

Article

Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

Inserts

hybrid GFP
mKate2

Use

E. coli cell free protein synthesis extracts

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2139

Plasmid

#162132

Purpose

Expression and Complementation plasmid for MlaD TM swap with LptC TM

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(TMdeltaLptC TM)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2138

Plasmid

#162133

Purpose

Expression and Complementation plasmid for MlaD TM swap with LetB TM

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(TMdeltaLetB TM)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL1198

Plasmid

#162134

Purpose

Expression and complementation plasmid for MlaFEDCB, with N-terminal his tag on MlaE

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2093

Plasmid

#162135

Purpose

Expression and Complementation plasmid for MlaE IF1 1-15 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-15)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2132

Plasmid

#162136

Purpose

Expression and Complementation plasmid for MlaE IF1 1-25 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-25)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2092

Plasmid

#162137

Purpose

Expression and Complementation plasmid for MlaE IF1 1-30 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-30)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2133

Plasmid

#162138

Purpose

Expression and Complementation plasmid for MlaE IF1 1-39 aa deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE(delta1-39)-(6xHis-MlaD)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Avs4_Chimera

Plasmid

#188802

Purpose

Chimeric Avs4 with transmembrane effector

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Avs4 chimera

Expression

Bacterial

Promoter

EcAvs4 native promoter

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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p-preSufI(IAC)

Plasmid

#168516

Purpose

Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

pre-SufI(IAC)

Tags

6xHisC tag and HSV tag

Expression

Bacterial

Mutation

C17I and C295A

Promoter

T7

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEc-crRNA2

Plasmid

#170089

Purpose

encodes E. coli type I-E single spacer array

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E single spacer array

Use

CRISPR and Synthetic Biology

Promoter

J23119

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2039

Plasmid

#139929

Purpose

Complementation plasmid for LetAB without LetB TM region

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(43-877)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2005

Plasmid

#139880

Purpose

Complementation plasmid for LetAB with 2 ring (rings17) LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(delta161-747)

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1993

Plasmid

#139897

Purpose

Expression plasmid for LetB PLL1 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta114-128, P113G)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1999

Plasmid

#139903

Purpose

Expression plasmid for LetB PLL7 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta816-826, S815G, T827G)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHIS8-3_DL121

Plasmid

#171953

Purpose

Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Insert

Fusion of ecDHFR and asLOV2 at position 121 of DHFR

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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