We narrowed to 7,077 results for: GFP expression plasmids

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pAAV-Syn-Chronos-GFP

Plasmid

#59170

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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1M-Qt-FLAG-TGA-rt20s-antiGFP

Plasmid

#206953

Purpose

Mammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, QtEnc, antiGFP-intrabody

Expression

Mammalian

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChrimsonR-GFP

Plasmid

#58806

Purpose

AAV expression of ChrimsonR-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

K176R

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pSMT-MjtRNA-GFP(47TAG)

Plasmid

#193251

Purpose

Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)

Depositor

Peter Schultz, Jessica Seeliger

Article

Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

Inserts

GFP(47TAG)
MjtRNA

Expression

Bacterial

Mutation

Codon for Tyr47 replaced with amber stop codon TA…

Promoter

hsp60 and metU

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Chronos-GFP

Plasmid

#58805

Purpose

AAV expression of Chronos-GFP under the CaMKII promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CoChR-GFP

Plasmid

#59070

Purpose

AAV-mediated expression of CoChR-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CoChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pIZ-EGFP-MYO10-HMM

Plasmid

#87257

Purpose

Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Expression

Insect

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

OpIE2

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2-mt2

Plasmid

#220245

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2 (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

Promoter

EF-1α

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_DmKHC[1-421]-1xGFP-6xHis

Plasmid

#196972

Purpose

Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

Depositor

Lukas Kapitein

Article

Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

Insert

Kinesin Heavy Chain (Khc Fly)

Tags

6xHis and GFP

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2-mt1

Plasmid

#220244

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 1 (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R27A, R31A, F165A

Promoter

EF-1α

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-FLEX-rc[Chronos-GFP]

Plasmid

#59056

Purpose

Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-HA-Slc1a5 (mouse)

Plasmid

#71458

Purpose

inducible expression fo Slc1a5 in mammalian cells

Depositor

Shao-Cong Sun

Article

Nakaya et al Immunity. 2014 May 15;40(5):692-705. doi

Insert

Slc1a5 (Slc1a5 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

A85P, L125F and V384A mutations compared to GenBa…

Promoter

CMV

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only
pHIV-EGFP.Flag-EZH2-mt2*

Plasmid

#220246

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2* (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

PRKKKR494-499NAAIRS

Promoter

EF-1α

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CsChR-GFP

Plasmid

#58841

Purpose

AAV expression of CsChR-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CsChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1093-1317_W

Plasmid

#147892

Purpose

Mammalian Expression of HsNot1iso1_1093-1317 *Please note that this plasmid encodes amino acids 1093-1322 (not 1093-1317 as the name suggests).

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1093-1317 (CNOT1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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LV-hGFAP-GFP-T2A-SOX2

Plasmid

#183908

Purpose

Expression of GFP and human SOX2 under the human GFAP ABC1D promoter

Depositor

Chun-Li Zhang

Article

Niu et al Nat Cell Biol. 2013 Oct;15(10):1164-75.

Insert

SOX2 (GFAP, SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-APLP1ICD-IRES-hrGFP

Plasmid

#107546

Purpose

AAV-mediated expression of last 50 amino acids of APLP1 protein (APLP1ICD) and IRES-mediated co-expression of hrGFP to recognize labelled cells

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

APLP1ICD (APLP1 Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pGA1-EF1α-sfGFP150TAG-4xtRNA

Plasmid

#251517

Purpose

Expression of sfGFP150TAG using the Methanomethylophilus alvus (Ma) PylRS/tRNA system for site-specific incorporation of noncanonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021

Insert

sfGFP150TAG

Tags

His6 and V5

Expression

Mammalian

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

EF1alpha

Available Since

March 12, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL3-01.ChR2(H134R)-GFP.BCC10

Plasmid

#229639

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-01.ChR2(H134R)-GFP.BCC10

Use

AAV

Expression

Mammalian

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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Qt-FLAG-TGA-rt9us-antiGFP

Plasmid

#206954

Purpose

Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

QtEnc, antiGFP-intrabody

Expression

Mammalian

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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