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pCMV HA hRB delta CDK + T821

Plasmid

#58920

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T821 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T821 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T826

Plasmid

#58921

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T826 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T826 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S249

Plasmid

#58908

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S249 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S249 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-Flag-EcoCas6

Plasmid

#106274

Purpose

Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasE

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas6-KRAB-2a-BlastR

Plasmid

#126492

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6-KRAB-2a-BlastR

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas8e

Plasmid

#106270

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasA

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCse2

Plasmid

#106271

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasB

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas7

Plasmid

#106272

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasC

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas5

Plasmid

#106273

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasD

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRb delta CDK + S230

Plasmid

#58907

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S230 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T252

Plasmid

#58910

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T252 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
YAF2_pLX307

Plasmid

#98383

Purpose

Lentiviral expression of YAF2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

YAF2 (YAF2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pWzl_neo_DEST_flag_PIK3CA

Plasmid

#45308

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
TLK1_pLX307

Plasmid

#98378

Purpose

Lentiviral expression of TLK1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TLK1 (TLK1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_ARPM1

Plasmid

#45259

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

ARPM1 (ACTRT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
MTS-GRSF1(IDR)-mCh-CRY2olig

Plasmid

#254614

Purpose

Expresses mt-optoIDR with GRSF1(1-150 aa) to optogenetically induce biomolecular condensates within mitochondrial matrix in living cells.

Depositor

Marina Feric

Article

Aththawala Gedara et al Commun Biol. 2026 Apr 23. doi: 10.1038/s

Insert

GRSF1 (GRSF1 Synthetic, Human)

Use

Synthetic Biology

Tags

mCherry-Cry2olig

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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hTRAK1(S719A, S919A)

Plasmid

#225226

Purpose

Expresses MYC-tagged hTRAK1(S719A, S919A)

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

trak1 (TRAK1 Human)

Tags

MYC

Expression

Mammalian

Mutation

S719A, S919A mutations

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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MYC-hTRAK1(S919A)

Plasmid

#219570

Purpose

Expresses MYC-tagged hTRAK1 (S919A)

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

trak1 (TRAK1 Human)

Tags

MYC

Expression

Mammalian

Mutation

Serine 919 changed to alanine.

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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MYC-hTRAK1(S719A)

Plasmid

#219571

Purpose

Expresses MYC-tagged hTRAK1 (S719A)

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

trak1 (TRAK1 Human)

Tags

MYC

Expression

Mammalian

Mutation

Changed serine 719 to alanine

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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MYC-hTRAK1(T834A)

Plasmid

#219573

Purpose

Expresses MYC-tagged hTRAK1 (T834A)

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

trak1 (TRAK1 Human)

Tags

MYC

Expression

Mammalian

Mutation

Serine 834 changed to alanine

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge