We narrowed to 24,746 results for: fer

1. gRNA_SRR134.3_miRFP670

   Plasmid

   #163754

   Purpose

   gRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   gRNA_SRR134.3
   

   Use

   CRISPR

   Tags

   miRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDLterm

   Plasmid

   #211903

   Purpose

   Base plasmid for dual-luciferase constructs to measure terminator strength

   Depositor

   Stan Fields

   Article

   Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S enhancer + 35S minimal promoter

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRS-dCas9

   Plasmid

   #220193

   Purpose

   mini-Tn7 dCas9 expression vector

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   promoterless

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQA-LP580

   Plasmid

   #222959

   Purpose

   Expresses R141S K142S LoaP double mutant

   Depositor

   Adrian Ferré-D'Amaré

   Article

   Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

   Insert

   antiterminator protein LoaP
   

   Use

   Tags

   10xHIS-SUMO

   Expression

   Bacterial

   Mutation

   mutated Arginine 141 to Serine and Lysine 142 to …

   Promoter

   T7

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQA-LP579

   Plasmid

   #222958

   Purpose

   Expresses R36S K51S LoaP double mutant

   Depositor

   Adrian Ferré-D'Amaré

   Article

   Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

   Insert

   antiterminator protein LoaP
   

   Use

   Tags

   10xHIS-SUMO

   Expression

   Bacterial

   Mutation

   mutated Arginine 36 to Serine and Lysine 51 to Se…

   Promoter

   T7

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQA-LP578

   Plasmid

   #222957

   Purpose

   Expresses R144S LoaP

   Depositor

   Adrian Ferré-D'Amaré

   Article

   Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

   Insert

   antiterminator protein LoaP
   

   Use

   Tags

   10xHIS-SUMO

   Expression

   Bacterial

   Mutation

   mutated Arginine 144 to Serine

   Promoter

   T7

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQA-LP577

   Plasmid

   #222956

   Purpose

   Expresses K142S LoaP

   Depositor

   Adrian Ferré-D'Amaré

   Article

   Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

   Insert

   antiterminator protein LoaP
   

   Use

   Tags

   10xHIS-SUMO

   Expression

   Bacterial

   Mutation

   mutated Lysine 142 to Serine

   Promoter

   T7

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQA-LP576

   Plasmid

   #222955

   Purpose

   Expresses R141S LoaP

   Depositor

   Adrian Ferré-D'Amaré

   Article

   Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

   Insert

   antiterminator protein LoaP
   

   Use

   Tags

   10xHIS-SUMO

   Expression

   Bacterial

   Mutation

   mutated Arginine 141 to Serine

   Promoter

   T7

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQA-LP575

   Plasmid

   #222954

   Purpose

   Expresses R36S LoaP

   Depositor

   Adrian Ferré-D'Amaré

   Article

   Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

   Insert

   antiterminator protein LoaP
   

   Use

   Tags

   10xHIS-SUMO

   Expression

   Bacterial

   Mutation

   mutated Arginine 36 to Serine

   Promoter

   T7

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQA-LP573

    Plasmid

    #222952

    Purpose

    Expresses E40H LoaP

    Depositor

    Adrian Ferré-D'Amaré

    Article

    Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

    Insert

    antiterminator protein LoaP
    

    Use

    Tags

    10xHIS-SUMO

    Expression

    Bacterial

    Mutation

    mutated Glutamate 40 to Histidine

    Promoter

    T7

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MARS-nGFP

    Plasmid

    #205234

    Purpose

    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
    

    Use

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3-2xMARS-PLD

    Plasmid

    #205237

    Purpose

    Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF

    Depositor

    Jeremy Baskin

    Inserts

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    phospholipase D (PLD)
    

    Use

    Tags

    Nuclear Export Sequence and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQA-LP572

    Plasmid

    #222951

    Purpose

    Expresses E40A LoaP

    Depositor

    Adrian Ferré-D'Amaré

    Article

    Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

    Insert

    antiterminator protein LoaP
    

    Use

    Tags

    10xHIS-SUMO

    Expression

    Bacterial

    Mutation

    mutated Glutamate 40 to Alanine

    Promoter

    T7

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. POC1478

    Plasmid

    #213804

    Purpose

    BsaI-associated switch methylase subunit S

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

    Insert

    BsaI-associated switch methylase S.Sen0738I
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. POC1476

    Plasmid

    #213802

    Purpose

    BsaI-associated switch methylase subunit M

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

    Insert

    BsaI-associated switch methylase M.Sen0738I
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. POC1477

    Plasmid

    #213803

    Purpose

    BsaI-associated switch methylase subunit S

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

    Insert

    BsaI-associated switch methylase S.Sen0738I
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. POC1475

    Plasmid

    #213801

    Purpose

    BsaI-associated switch methylase subunit M

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

    Insert

    BsaI-associated switch methylase M.Sen0738I
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Noodles Plasmid

    Plasmid

    #212613

    Purpose

    An all-in-one system for on-target efficiency analysis of CRISPR guide RNAs

    Depositor

    Ilya Vinnikov

    Article

    Lin et al MethodsX. 2023 Nov 15;12:102481. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Modular BE4max for GG insertion

    Plasmid

    #198891

    Purpose

    Easily insert deaminase of choice into BE4max using Golden Gate cloning

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    CRISPR associated protein 9 (cas9 Streptococcus pyogenes)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 NKX6-2-Venus S246A

    Plasmid

    #203715

    Purpose

    human NKX6-2 S246A phosphoresistant mutant

    Depositor

    Federico Herrera

    Article

    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

    Insert

    NKX6-2 (S246A) fused with Venus (NKX6-2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    NKX6-2 (S246A)

    Promoter

    CMV

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge